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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene
Year:
2013
Authors :
אשד, רוית
;
.
באומקולר, פביאן
;
.
בורגר, יוסף
;
.
בר, עינת
;
.
גלפז, נבות
;
.
לביא, תמר
;
.
לוינסון, אפרים
;
.
מאיר, איילה
;
.
מלמד, טל
;
.
סער, יובל
;
.
סער, עוזי
;
.
פדר, ארי
;
.
פורטנוי, ויטלי
;
.
צורי, גליל
;
.
קציר, נורית
;
.
שור-שמעוני, עינב
;
.
שפר, ארתור
;
.
שרמן, עמיר
;
.
תדמור, יעקב
;
.
Volume :
539
Co-Authors:
Galpaz, N., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Burger, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Lavee, T., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Tzuri, G., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Sherman, A., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Melamed, T., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Eshed, R., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Meir, A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Portnoy, V., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Bar, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Shimoni-Shor, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Feder, A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Saar, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Saar, U., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Baumkoler, F., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Lewinsohn, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Schaffer, A.A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Katzir, N., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Tadmor, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Facilitators :
From page:
117
To page:
125
(
Total pages:
9
)
Abstract:
In order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'Charentais' type melon line that accumulates β-carotene. One mutagenized M2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofI'). HPLC analysis revealed that 'yofI' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. The altered carotenoid composition of 'yofI' is associated with a significant change of the fruit aroma since cleavage of β-carotene yields different apocarotenoids than the cleavage of pro-lycopene. Normally, pro-lycopene is further isomerized by CRTISO (carotenoid isomerase) to yield all-trans-lycopene, which is further cyclized to β-carotene in melon fruit. Cloning and sequencing of 'yofI' CRTISO identified two mRNA sequences which lead to truncated forms of CRTISO. Sequencing of the genomic CRTISO identified an A-T transversion in 'yofI' which leads to a premature STOP codon. The early carotenoid pathway genes were up regulated in yofI fruit causing accumulation of other intermediates such as phytoene and ζ-carotene. Total carotenoid levels are only slightly increased in the mutant. Mutants accumulating pro-lycopene have been reported in both tomato and watermelon fruits, however, this is the first report of a non-lycopene accumulating fruit showing this phenomenon. © 2013 Elsevier Inc. All rights reserved.
Note:
Related Files :
biosynthesis
chlorophyll
Cucumis melo
gene expression
Genetics
metabolism
phenotype
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.abb.2013.08.006
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28181
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
You may also be interested in
Scientific Publication
Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene
539
Galpaz, N., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Burger, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Lavee, T., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Tzuri, G., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Sherman, A., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Melamed, T., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Eshed, R., Department of Fruit Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Meir, A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Portnoy, V., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Bar, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Shimoni-Shor, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Feder, A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Saar, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Saar, U., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Baumkoler, F., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Lewinsohn, E., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Schaffer, A.A., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Katzir, N., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Tadmor, Y., Department of Vegetable Crops and Plant Genetics, Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay 30-095, Israel
Genetic and chemical characterization of an EMS induced mutation in Cucumis melo CRTISO gene
In order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'Charentais' type melon line that accumulates β-carotene. One mutagenized M2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofI'). HPLC analysis revealed that 'yofI' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. The altered carotenoid composition of 'yofI' is associated with a significant change of the fruit aroma since cleavage of β-carotene yields different apocarotenoids than the cleavage of pro-lycopene. Normally, pro-lycopene is further isomerized by CRTISO (carotenoid isomerase) to yield all-trans-lycopene, which is further cyclized to β-carotene in melon fruit. Cloning and sequencing of 'yofI' CRTISO identified two mRNA sequences which lead to truncated forms of CRTISO. Sequencing of the genomic CRTISO identified an A-T transversion in 'yofI' which leads to a premature STOP codon. The early carotenoid pathway genes were up regulated in yofI fruit causing accumulation of other intermediates such as phytoene and ζ-carotene. Total carotenoid levels are only slightly increased in the mutant. Mutants accumulating pro-lycopene have been reported in both tomato and watermelon fruits, however, this is the first report of a non-lycopene accumulating fruit showing this phenomenon. © 2013 Elsevier Inc. All rights reserved.
Scientific Publication
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