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פותח על ידי קלירמאש פתרונות בע"מ -
Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro
Year:
2003
Source of publication :
Plant Cell Reports
Authors :
וישנבצקי, ג'יין
;
.
Volume :
21
Co-Authors:
Vishnevetsky, J., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Zamski, E., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Ziv, M., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Facilitators :
From page:
645
To page:
650
(
Total pages:
6
)
Abstract:
Nerine (Nerine sarniensis) cv. Salmon Supreme in vitro-grown bulblets, 7-9 mm in diameter, were cut in half longitudinally and used for adventitious bud initiation following dissection of the roots and two-thirds of the upper part of the bulblets. The terminal apex was injured with a hot, sterile microscope dissecting needle. The highest number of buds formed (seven to nine buds per halved bulblet) on a semi-solid Murashige and Skoog (MS) basal salts medium supplemented with 3% sucrose and either 1 μM 6-benzylaminopurine (BA) and 1 μM α-naphthaleneacetic acid (NAA) or 0.5 μM BA and 0.1 μM NAA. Bulblet halves were cultured in the dark for 11-13 weeks with one subculture after 6 weeks. Anatomical studies indicated that the initiation of adventitious buds on the abaxial side of the inner scales of the halved bulblet was adjacent to the basal plate and started from the leaf primordium and a meristematic bulge. Buds developed directly into small bulblets after they were transferred to semi-solid MS basal salts medium supplemented with 6% sucrose, 10 μM indole-3-butyric acid (IBA) and 0.25% activated charcoal. Small bulblets cultured in liquid MS medium supplemented with additional KH2PO4 (170 mg 1-1), 6% sucrose and 0.1 μM NAA under a 16/8-h (light/dark) photoperiod for 8 weeks grew into larger bulbs faster than those cultured on semi-solid medium. The bulbs were rooted on a semi-solid medium after 4 weeks and then transferred to the soil. As many as 18 bulblets developed and rooted from one in vitro-grown bulb after 25-27 weeks.
Note:
Related Files :
Activated carbon
Adventitious buds
Basalt
Bublet regeneration
Bulbs
liquid culture
Naphthaleneacetic Acids
Propagation
Soils
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28213
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
Scientific Publication
Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro
21
Vishnevetsky, J., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Zamski, E., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Ziv, M., Department of Agricultural Botany, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Enhanced bud and bulblet regeneration from bulbs of Nerine sarniensis cultured in vitro
Nerine (Nerine sarniensis) cv. Salmon Supreme in vitro-grown bulblets, 7-9 mm in diameter, were cut in half longitudinally and used for adventitious bud initiation following dissection of the roots and two-thirds of the upper part of the bulblets. The terminal apex was injured with a hot, sterile microscope dissecting needle. The highest number of buds formed (seven to nine buds per halved bulblet) on a semi-solid Murashige and Skoog (MS) basal salts medium supplemented with 3% sucrose and either 1 μM 6-benzylaminopurine (BA) and 1 μM α-naphthaleneacetic acid (NAA) or 0.5 μM BA and 0.1 μM NAA. Bulblet halves were cultured in the dark for 11-13 weeks with one subculture after 6 weeks. Anatomical studies indicated that the initiation of adventitious buds on the abaxial side of the inner scales of the halved bulblet was adjacent to the basal plate and started from the leaf primordium and a meristematic bulge. Buds developed directly into small bulblets after they were transferred to semi-solid MS basal salts medium supplemented with 6% sucrose, 10 μM indole-3-butyric acid (IBA) and 0.25% activated charcoal. Small bulblets cultured in liquid MS medium supplemented with additional KH2PO4 (170 mg 1-1), 6% sucrose and 0.1 μM NAA under a 16/8-h (light/dark) photoperiod for 8 weeks grew into larger bulbs faster than those cultured on semi-solid medium. The bulbs were rooted on a semi-solid medium after 4 weeks and then transferred to the soil. As many as 18 bulblets developed and rooted from one in vitro-grown bulb after 25-27 weeks.
Scientific Publication
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