נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Detection of Erwinia herbicola pv. gypsophilae in gypsophila plants by PCR
Year:
1998
Authors :
דרור, אורית
;
.
קוגן, נינה
;
.
קלייטמן, פרידה
;
.
Volume :
104
Co-Authors:
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kogan, N., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Valinsky, L., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dror, O., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kleitman, F., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
85
To page:
91
(
Total pages:
7
)
Abstract:
Three PCR primer pairs, based on the cytokinins (etz) or IAA biosynthetic genes, were used for detecting Erwinia herbicola pv. gypsophilae in Gypsophila paniculata plants. The primers were specific to all gall-forming E. herbicola strains and distinguished them from saprophytic strains associated with gypsophila plants or from other gall-forming bacteria. In pure culture of the pathogen, less than one bacterial cell was detected with nested PCR using the etz primers - an increase of 100-fold in sensitivity as compared with single-round PCR. In the presence of plant extract a reduction of tenfold in sensitivity was observed by nested PCR. When cells were grown on a semi-selective medium prior to PCR (Bio-PCR), five cells from pure culture of the pathogen were detected. The bacteria could be detected by nested-PCR or Bio-PCR in symptomless gypsophila cuttings after 7 days. The Bio-PCR procedure described in this study can be used to establish disease-free nuclear stock of mother plants of gypsophila.
Note:
Related Files :
Bio-PCR
diagnosis
Disease detection
Erwinia herbicola
galls
Gypsophila paniculata
Nested-PCR
Polymerase Chain Reaction
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1008659001313
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28220
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
You may also be interested in
Scientific Publication
Detection of Erwinia herbicola pv. gypsophilae in gypsophila plants by PCR
104
Manulis, S., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kogan, N., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Valinsky, L., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dror, O., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kleitman, F., Department of Plant Pathology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Detection of Erwinia herbicola pv. gypsophilae in gypsophila plants by PCR
Three PCR primer pairs, based on the cytokinins (etz) or IAA biosynthetic genes, were used for detecting Erwinia herbicola pv. gypsophilae in Gypsophila paniculata plants. The primers were specific to all gall-forming E. herbicola strains and distinguished them from saprophytic strains associated with gypsophila plants or from other gall-forming bacteria. In pure culture of the pathogen, less than one bacterial cell was detected with nested PCR using the etz primers - an increase of 100-fold in sensitivity as compared with single-round PCR. In the presence of plant extract a reduction of tenfold in sensitivity was observed by nested PCR. When cells were grown on a semi-selective medium prior to PCR (Bio-PCR), five cells from pure culture of the pathogen were detected. The bacteria could be detected by nested-PCR or Bio-PCR in symptomless gypsophila cuttings after 7 days. The Bio-PCR procedure described in this study can be used to establish disease-free nuclear stock of mother plants of gypsophila.
Scientific Publication
You may also be interested in