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פותח על ידי קלירמאש פתרונות בע"מ -
Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control
Year:
1994
Source of publication :
Journal of Molecular Biology
Authors :
גפני, רון
;
.
Volume :
243
Co-Authors:
Gafny, R., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet-Dagan, 50250, Israel
Cohen, S., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Nachaliel, N., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Glaser, G., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Facilitators :
From page:
152
To page:
156
(
Total pages:
5
)
Abstract:
Ribosomal RNA synthesis in Escherichia coli is under stringent control. The seven rRNA operons are highly conserved and are each transcribed from two tandem promoters, P1 and P2, which are located about 120 base-pairs apart. In exponentially growing cells the majority of the transcripts are initiated at the P1 promoters. The P1 promoters are highly regulated, and are under stringent as well as growth rate controls. Here we demonstrate that transcription from the rrnA P1 promoter diminishes P2 expression. In the absence of P1, the P2 promoter acts as a rather strong promoter. Insertion of a transcription terminator between P1 and P2 eliminates the inhibition of P2 by P1, suggesting that the physical movement of RNA polymerase originating at P1 and progressing along the P2 promoter is necessary for the interference process to take place. Similarly to P1, the solitary P2 promoter is subject to stringent control.
Note:
Related Files :
Base Sequence
guanosine
Molecular Sequence Data
RNA, Ribosomal
Stringent control
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/jmbi.1994.1641
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28226
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:37
Scientific Publication
Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control
243
Gafny, R., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel, Department of Virology, Agricultural Research Organization, Volcani Center, Bet-Dagan, 50250, Israel
Cohen, S., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Nachaliel, N., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Glaser, G., Department of Cellular Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control
Ribosomal RNA synthesis in Escherichia coli is under stringent control. The seven rRNA operons are highly conserved and are each transcribed from two tandem promoters, P1 and P2, which are located about 120 base-pairs apart. In exponentially growing cells the majority of the transcripts are initiated at the P1 promoters. The P1 promoters are highly regulated, and are under stringent as well as growth rate controls. Here we demonstrate that transcription from the rrnA P1 promoter diminishes P2 expression. In the absence of P1, the P2 promoter acts as a rather strong promoter. Insertion of a transcription terminator between P1 and P2 eliminates the inhibition of P2 by P1, suggesting that the physical movement of RNA polymerase originating at P1 and progressing along the P2 promoter is necessary for the interference process to take place. Similarly to P1, the solitary P2 promoter is subject to stringent control.
Scientific Publication
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