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The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity
Year:
2014
Source of publication :
Development (Cambridge)
Authors :
קוטשר, יערית
;
.
Volume :
141
Co-Authors:
Mandel, T., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Moreau, F., CNRS, UMR5168, F-38054 Grenoble, France
Kutsher, Y., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Fletcher, J.C., Plant Gene Expression Center, USDA-ARS/University of California, Berkeley, 800 Buchanan Street, Albany, CA 94710, United States
Carles, C.C., CNRS, UMR5168, F-38054 Grenoble, France
Williams, L.E., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Facilitators :
From page:
830
To page:
841
(
Total pages:
12
)
Abstract:
In plants, the shoot apical meristem (SAM) serves as a reservoir of pluripotent stem cells from which all above ground organs originate. To sustain proper growth, the SAM must maintain homeostasis between the self-renewal of pluripotent stem cells and cell recruitment for lateral organ formation. At the core of the network that regulates this homeostasis in Arabidopsis are the WUSCHEL (WUS) transcription factor specifying stem cell fate and the CLAVATA (CLV) ligand-receptor system limiting WUS expression. In this study, we identified the ERECTA (ER) pathway as a second receptor kinase signaling pathway that regulates WUS expression, and therefore shoot apical and floral meristem size, independently of the CLV pathway. We demonstrate that reduction in class III HD-ZIP and ER function together leads to a significant increase in WUS expression, resulting in extremely enlarged shoot meristems and a switch from spiral to whorled vegetative phyllotaxy. We further show that strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell, suggesting an indirect role for ER in regulating floral meristem identity. This work illustrates the delicate balance between stem cell specification and differentiation in the meristem and shows that a shift in this balance leads to abnormal phyllotaxy and to altered reproductive cell fate. © 2014. Published by The Company of Biologists Ltd.
Note:
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עוד תגיות
תוכן קשור
More details
DOI :
10.1242/dev.104687
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28281
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
You may also be interested in
Scientific Publication
The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity
141
Mandel, T., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Moreau, F., CNRS, UMR5168, F-38054 Grenoble, France
Kutsher, Y., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
Fletcher, J.C., Plant Gene Expression Center, USDA-ARS/University of California, Berkeley, 800 Buchanan Street, Albany, CA 94710, United States
Carles, C.C., CNRS, UMR5168, F-38054 Grenoble, France
Williams, L.E., Food and Environment, The Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel
The ERECTA receptor kinase regulates Arabidopsis shoot apical meristem size, phyllotaxy and floral meristem identity
In plants, the shoot apical meristem (SAM) serves as a reservoir of pluripotent stem cells from which all above ground organs originate. To sustain proper growth, the SAM must maintain homeostasis between the self-renewal of pluripotent stem cells and cell recruitment for lateral organ formation. At the core of the network that regulates this homeostasis in Arabidopsis are the WUSCHEL (WUS) transcription factor specifying stem cell fate and the CLAVATA (CLV) ligand-receptor system limiting WUS expression. In this study, we identified the ERECTA (ER) pathway as a second receptor kinase signaling pathway that regulates WUS expression, and therefore shoot apical and floral meristem size, independently of the CLV pathway. We demonstrate that reduction in class III HD-ZIP and ER function together leads to a significant increase in WUS expression, resulting in extremely enlarged shoot meristems and a switch from spiral to whorled vegetative phyllotaxy. We further show that strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell, suggesting an indirect role for ER in regulating floral meristem identity. This work illustrates the delicate balance between stem cell specification and differentiation in the meristem and shows that a shift in this balance leads to abnormal phyllotaxy and to altered reproductive cell fate. © 2014. Published by The Company of Biologists Ltd.
Scientific Publication
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