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פותח על ידי קלירמאש פתרונות בע"מ -
Co-integration of β-lactoglobulin/human serum albumin hybrid genes with the entire β-lactoglobulin gene or the matrix attachment region element: Repression of human serum albumin and β-lactoglobulin expression in the mammary gland and dual regulation of the transgenes
Year:
1996
Authors :
ברש, איתמר
;
.
קארי, רחל
;
.
שני, משה
;
.
Volume :
45
Co-Authors:
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Kari, R., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Hurwitz, D.R., Rhone Poulence Rorer Ctrl. Research, P.O. Box 1200, Collegeville, PA, United States
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
421
To page:
430
(
Total pages:
10
)
Abstract:
The effect of co-integration of the entire β-lactoglobulin (BLG) gene or matrix attachment region (MAR) sequences on the expression of various BLG/human serum albumin (HSA) gene constructs was tested in transgenic mice. These former sequences were chosen because of their reported ability to insulate transgenes from the neighboring host genomic DNA sequences and/or to provide a more permissive transcriptional environment. When introduced alone, a cDNA-based BLG/HSA construct was expressed in 60% of transgenic strains and HSA was secreted at levels up to 0.3 mg/ml into the milk. Upon co-integration with either the entire BLG gene or MAR element, HSA RNA and protein expression were completely abrogated. While the co-integrated BLG gene suppressed the proportion of expresser strains carrying cDNA as well as genomic BLG/HSA constructs, the MAR element only exerted its negative effect on the cDNA-based BLG/HSA construct. In transgenics expressing both HSA and BLG, the tissue specificity and developmental patterns of BLG expression were altered and resembled the less stringent pattern of the BLG/HSA expression. These results demonstrate that rescue of transgene expression through co- integration with BLG or MAR sequences do not apply universally.
Note:
Related Files :
Animals
animal tissue
gene expression
Genome
lactation
mice
milk
Nuclear Matrix
RNA
sheep
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/(SICI)1098-2795(199612)45:4<421::AID-MRD3>3.0.CO;2-T
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28373
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
You may also be interested in
Scientific Publication
Co-integration of β-lactoglobulin/human serum albumin hybrid genes with the entire β-lactoglobulin gene or the matrix attachment region element: Repression of human serum albumin and β-lactoglobulin expression in the mammary gland and dual regulation of the transgenes
45
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Kari, R., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Hurwitz, D.R., Rhone Poulence Rorer Ctrl. Research, P.O. Box 1200, Collegeville, PA, United States
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Co-integration of β-lactoglobulin/human serum albumin hybrid genes with the entire β-lactoglobulin gene or the matrix attachment region element: Repression of human serum albumin and β-lactoglobulin expression in the mammary gland and dual regulation of the transgenes
The effect of co-integration of the entire β-lactoglobulin (BLG) gene or matrix attachment region (MAR) sequences on the expression of various BLG/human serum albumin (HSA) gene constructs was tested in transgenic mice. These former sequences were chosen because of their reported ability to insulate transgenes from the neighboring host genomic DNA sequences and/or to provide a more permissive transcriptional environment. When introduced alone, a cDNA-based BLG/HSA construct was expressed in 60% of transgenic strains and HSA was secreted at levels up to 0.3 mg/ml into the milk. Upon co-integration with either the entire BLG gene or MAR element, HSA RNA and protein expression were completely abrogated. While the co-integrated BLG gene suppressed the proportion of expresser strains carrying cDNA as well as genomic BLG/HSA constructs, the MAR element only exerted its negative effect on the cDNA-based BLG/HSA construct. In transgenics expressing both HSA and BLG, the tissue specificity and developmental patterns of BLG expression were altered and resembled the less stringent pattern of the BLG/HSA expression. These results demonstrate that rescue of transgene expression through co- integration with BLG or MAR sequences do not apply universally.
Scientific Publication
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