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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of Alternaria alternata glucanase genes expressed during infection of resistant and susceptible persimmon fruits
Year:
2002
Source of publication :
Molecular Plant Pathology
Authors :
אשל, דני
;
.
ליכטר, אמנון
;
.
פרוסקי, דב
;
.
Volume :
3
Co-Authors:
Eshel, D., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lichter, A., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dinoor, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
347
To page:
358
(
Total pages:
12
)
Abstract:
Preharvest treatment with gibberellic acid (GA3) or its inhibitor paclobutrazol (PBZ) can reduce or increase, respectively, the susceptibility of persimmon fruits to Alternaria alternata. This was suggested to be the result of the ability of the fungus and produced endoglucanases to induce symptom development. To evaluate the importance of glucanases during A. alternata attack, five glucanase genes, corresponding to the C, F, and K families, were cloned from A. alternata using 'family-specific' oligonucleotide primers. The genes, present in a single copy, encode for exoglucanases AaC1 and AaC2, endoxylanase AaF1, endoglucanase AaK1, and the mixed-linked glucanase AaMLG1. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis of RNA extracted from persimmon fruits, 2 and 4 days post-infection with A, alternata, showed the expression of all five glucanase genes in GA3- and PBZ-treated fruits. However, transcription levels and enzyme production of the endoglucanase (AaK1) and one exoglucanase (AaC1) were enhanced during A. alternata growth on cell walls from susceptible PBZ-treated fruits, whereas the expression of these genes and their enzyme production were significantly reduced in resistant GA3-treated fruits. The present results suggest the involvement of endo- and exoglucanase in symptom development caused by A. alternata in resistant and susceptible persimmon fruits.
Note:
Related Files :
Alternaria
Alternaria
Alternaria alternata
Diospyros kaki
Diospyros virginiana
Eukaryota
fungi
Myxogastria
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1364-3703.2002.00127.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28398
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:38
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Scientific Publication
Characterization of Alternaria alternata glucanase genes expressed during infection of resistant and susceptible persimmon fruits
3
Eshel, D., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Lichter, A., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dinoor, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, Institute for Technology and Storage of Agricultural Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Characterization of Alternaria alternata glucanase genes expressed during infection of resistant and susceptible persimmon fruits
Preharvest treatment with gibberellic acid (GA3) or its inhibitor paclobutrazol (PBZ) can reduce or increase, respectively, the susceptibility of persimmon fruits to Alternaria alternata. This was suggested to be the result of the ability of the fungus and produced endoglucanases to induce symptom development. To evaluate the importance of glucanases during A. alternata attack, five glucanase genes, corresponding to the C, F, and K families, were cloned from A. alternata using 'family-specific' oligonucleotide primers. The genes, present in a single copy, encode for exoglucanases AaC1 and AaC2, endoxylanase AaF1, endoglucanase AaK1, and the mixed-linked glucanase AaMLG1. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis of RNA extracted from persimmon fruits, 2 and 4 days post-infection with A, alternata, showed the expression of all five glucanase genes in GA3- and PBZ-treated fruits. However, transcription levels and enzyme production of the endoglucanase (AaK1) and one exoglucanase (AaC1) were enhanced during A. alternata growth on cell walls from susceptible PBZ-treated fruits, whereas the expression of these genes and their enzyme production were significantly reduced in resistant GA3-treated fruits. The present results suggest the involvement of endo- and exoglucanase in symptom development caused by A. alternata in resistant and susceptible persimmon fruits.
Scientific Publication
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