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פותח על ידי קלירמאש פתרונות בע"מ -
Cloning of a full-length cDNA encoding an ethylene receptor ers homologue from citrus
Year:
2000
Source of publication :
Acta Horticulturae
Authors :
הולנד, דורון
;
.
Volume :
535
Co-Authors:
Li, C.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Zhong, G.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Jacob-Wilk, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
119
To page:
125
(
Total pages:
7
)
Abstract:
The gaseous phytohormone ethylene plays a very important role in regulating plant dormancy, growth, and development. An ethylene signal is perceived and responded to by an ethylene receptor protein family in Arabidopsis, and several genes from this family (ETR1, ERS, ETRl, ERS2 and EIN4) have been cloned. By utilizing Arabidopsis ETR\ gene as a molecular probe, a Citrus homologue was initially isolated from a Citrus (Citrus sinensis [L.] Osbeck cv. Valencia) genomic library and used to screen a cDNA library prepared from the same plant. The full length of the cDNA is 2400 bp with an open reading frame of 634 amino acid residues. The gene shares 69.1% nucleotide homology with ETR1, 73.3% with ERS, 72.4% with RP-ERS\, and 71.4% with NR of tomato. Based on sequence alignment analysis, Citrus-ERS appears to be a homologue of ERS. Both ERS and the Citrus-ERS homologues have an N-terminal ethylene-sensor domain and a histidine kinase domain, but lack a responsor domain found in the C-terminal of ETRl. The four amino acids that confer dominant ethylene insensitivity in the A. thaliana ETRl mutants are conserved.
Note:
Related Files :
arabidopsis
Arabidopsis thaliana
Gene cloning
Lycopersicon esculentum
signal transduction
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
28423
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
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Scientific Publication
Cloning of a full-length cDNA encoding an ethylene receptor ers homologue from citrus
535
Li, C.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Zhong, G.Y., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, 76100 Rehovot, Israel
Jacob-Wilk, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Institute of Horticulture, Agricultural Research Organization, P.O. Box 6, 50250 Bet Dagan, Israel
Cloning of a full-length cDNA encoding an ethylene receptor ers homologue from citrus
The gaseous phytohormone ethylene plays a very important role in regulating plant dormancy, growth, and development. An ethylene signal is perceived and responded to by an ethylene receptor protein family in Arabidopsis, and several genes from this family (ETR1, ERS, ETRl, ERS2 and EIN4) have been cloned. By utilizing Arabidopsis ETR\ gene as a molecular probe, a Citrus homologue was initially isolated from a Citrus (Citrus sinensis [L.] Osbeck cv. Valencia) genomic library and used to screen a cDNA library prepared from the same plant. The full length of the cDNA is 2400 bp with an open reading frame of 634 amino acid residues. The gene shares 69.1% nucleotide homology with ETR1, 73.3% with ERS, 72.4% with RP-ERS\, and 71.4% with NR of tomato. Based on sequence alignment analysis, Citrus-ERS appears to be a homologue of ERS. Both ERS and the Citrus-ERS homologues have an N-terminal ethylene-sensor domain and a histidine kinase domain, but lack a responsor domain found in the C-terminal of ETRl. The four amino acids that confer dominant ethylene insensitivity in the A. thaliana ETRl mutants are conserved.
Scientific Publication
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