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פותח על ידי קלירמאש פתרונות בע"מ -
TOMATO AGAMOUS-LIKE 1 is a component of the fruit ripening regulatory network
Year:
2009
Source of publication :
Plant Journal
Authors :
איטקין, מקסים
;
.
Volume :
60
Co-Authors:
Itkin, M., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Seybold, H., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel, Interfaculty Institute for Biochemistry, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Strasse 4, D-72076 Tübingen, Germany
Breitel, D., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Meir, S., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Facilitators :
From page:
1081
To page:
1095
(
Total pages:
15
)
Abstract:
After fertilization, the expanding carpel of fleshy fruit goes through a phase change to ripening. Although the role of ethylene signalling in mediating climacteric ripening has been established, knowledge regarding the regulation of ethylene biosynthesis and its association with fruit developmental programs is still lacking. A functional screen of tomato transcription factors showed that silencing of the TOMATO AGAMOUS-LIKE 1 (TAGL1) MADS box gene results in altered fruit pigmentation. Over-expressing TAGL1 as a chimeric repressor suggested a role in controlling ripening, as transgenic tomato fruit showed reduced carotenoid and ethylene levels, suppressed chlorophyll breakdown, and down-regulation of ripening-associated genes. Moreover, fruits over-expressing TAGL1 accumulated more lycopene, and their sepals were swollen, accumulated high levels of the yellow flavonoid naringenin chalcone and contained lycopene. Transient promoter-binding assays indicated that part of the TAGL1 activity in ripening is executed through direct activation of ACS2, an ethylene biosynthesis gene that has recently been reported to be a target of the RIN MADS box factor. Examination of the TAGL1 transcript and its over-expression in the rin mutant background suggested that RIN does not regulate TAGL1 or vice versa. The results also indicated RIN-dependent and-independent processes that are regulated by TAGL1. We also noted that fruit of TAGL1 loss-of-function lines had a thin pericarp layer, indicating an additional role for TAGL1 in carpel expansion prior to ripening. The results add a new component to the current model of the regulatory network that controls fleshy fruit ripening and its association with the ethylene biosynthesis pathway. © 2009 Weizmann Institute of Science. Journal compilation © 2009 Blackwell Publishing Ltd.
Note:
Related Files :
biosynthesis
carotenoids
ethylene
gene silencing
Genetics
metabolism
pigmentation
ripening
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2009.04064.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28502
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
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Scientific Publication
TOMATO AGAMOUS-LIKE 1 is a component of the fruit ripening regulatory network
60
Itkin, M., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Seybold, H., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel, Interfaculty Institute for Biochemistry, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Strasse 4, D-72076 Tübingen, Germany
Breitel, D., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Meir, S., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
TOMATO AGAMOUS-LIKE 1 is a component of the fruit ripening regulatory network
After fertilization, the expanding carpel of fleshy fruit goes through a phase change to ripening. Although the role of ethylene signalling in mediating climacteric ripening has been established, knowledge regarding the regulation of ethylene biosynthesis and its association with fruit developmental programs is still lacking. A functional screen of tomato transcription factors showed that silencing of the TOMATO AGAMOUS-LIKE 1 (TAGL1) MADS box gene results in altered fruit pigmentation. Over-expressing TAGL1 as a chimeric repressor suggested a role in controlling ripening, as transgenic tomato fruit showed reduced carotenoid and ethylene levels, suppressed chlorophyll breakdown, and down-regulation of ripening-associated genes. Moreover, fruits over-expressing TAGL1 accumulated more lycopene, and their sepals were swollen, accumulated high levels of the yellow flavonoid naringenin chalcone and contained lycopene. Transient promoter-binding assays indicated that part of the TAGL1 activity in ripening is executed through direct activation of ACS2, an ethylene biosynthesis gene that has recently been reported to be a target of the RIN MADS box factor. Examination of the TAGL1 transcript and its over-expression in the rin mutant background suggested that RIN does not regulate TAGL1 or vice versa. The results also indicated RIN-dependent and-independent processes that are regulated by TAGL1. We also noted that fruit of TAGL1 loss-of-function lines had a thin pericarp layer, indicating an additional role for TAGL1 in carpel expansion prior to ripening. The results add a new component to the current model of the regulatory network that controls fleshy fruit ripening and its association with the ethylene biosynthesis pathway. © 2009 Weizmann Institute of Science. Journal compilation © 2009 Blackwell Publishing Ltd.
Scientific Publication
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