חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Gene transfer into mammalian cells by jet injection
Year:
1995
Source of publication :
Hybridoma
Authors :
שמאי, אבי
;
.
Volume :
14
Co-Authors:
Furth, P.A., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Shamay, A., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Hennighausen, L., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Facilitators :
From page:
149
To page:
152
(
Total pages:
4
)
Abstract:
Jet injection of DNA in solution is a technique that can be used to transfer DNA into tissues of living animals, where the introduced genes are expressed. The muscle, fat, skin, and mammary tissue of mice, and the fat, skin, and mammary tissue of sheep can be transfected with DNA. A jet injector, such as the Ped-o-jet (Stirn Industries, Dayton, NJ), is used to form a jet from 100 to 300 μl of a DNA solution. This jet has sufficient force to travel into and through tissues of adult and juvenile animals. The introduced DNA is found in cells surrounding the path of the jet. When jet injection is performed through the surface of intact skin, underlying muscle, mammary, and fat, cells up to 2 cm distant from the point of injection are transfected with DNA. In this study, we demonstrate that the efficiency of DNA transfer is dependent upon the force of injection. Jet injection is an alternative to needle injection, lipofection, and particle bombardment for the introduction of 'naked' DNA into the tissues of animals. This technique has potential for the introduction of genes into living organisms for genetic vaccination and gene therapy.
Note:
Related Files :
Animal
Conference paper
DNA
Female
gene expression
gene therapy
Male
mice
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
28529
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
Scientific Publication
Gene transfer into mammalian cells by jet injection
14
Furth, P.A., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Shamay, A., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Hennighausen, L., VA Medical Center, Univ. of Maryland Medical School, 10 North Greene Street, Baltimore, MD 21201, United States
Gene transfer into mammalian cells by jet injection
Jet injection of DNA in solution is a technique that can be used to transfer DNA into tissues of living animals, where the introduced genes are expressed. The muscle, fat, skin, and mammary tissue of mice, and the fat, skin, and mammary tissue of sheep can be transfected with DNA. A jet injector, such as the Ped-o-jet (Stirn Industries, Dayton, NJ), is used to form a jet from 100 to 300 μl of a DNA solution. This jet has sufficient force to travel into and through tissues of adult and juvenile animals. The introduced DNA is found in cells surrounding the path of the jet. When jet injection is performed through the surface of intact skin, underlying muscle, mammary, and fat, cells up to 2 cm distant from the point of injection are transfected with DNA. In this study, we demonstrate that the efficiency of DNA transfer is dependent upon the force of injection. Jet injection is an alternative to needle injection, lipofection, and particle bombardment for the introduction of 'naked' DNA into the tissues of animals. This technique has potential for the introduction of genes into living organisms for genetic vaccination and gene therapy.
Scientific Publication
נגישות
menu      
You may also be interested in