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פותח על ידי קלירמאש פתרונות בע"מ -
Copy number variation of lipocalin family genes for male-specific proteins in tilapia and its association with gender
Year:
2008
Source of publication :
Heredity
Authors :
גוליק, מרינה
;
.
חולתא, גדעון
;
.
סרוסי, אייל
;
.
רון, מיכה
;
.
שיראק, אנדרי
;
.
Volume :
101
Co-Authors:
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Golik, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Lee, B.-Y., Department of Biology, University of Maryland, College Park, MD, United States
Howe, A.E., Department of Biology, University of Maryland, College Park, MD, United States
Kocher, T.D., Department of Biology, University of Maryland, College Park, MD, United States
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel, Institute of Animal Science, Agricultural Research Organization, Volcani Center, POB6, Beit Dagan 50250, Israel
Facilitators :
From page:
405
To page:
415
(
Total pages:
11
)
Abstract:
Lipocalins are involved in the binding of small molecules like sex steroids. We show here that the previously reported tilapia male-specific protein (MSP) is a lipocalin encoded by a variety of paralogous and homologous genes in different tilapia species. Exon-intron boundaries of MSP genes were typical of the six-exon genomic structure of lipocalins, and the transcripts were capable of encoding 200 amino-acid polypeptides that consisted of a putative signal peptide and a lipocalin domain. Cysteine residues are conserved in positions analogous to those forming the three disulfide bonds characteristic of the ligand pocket. The calculated molecular mass of the secreted MSP (20.4 kDa) was less than half of that observed, suggesting that it is highly glycosylated like its homologue tributyltin-binding protein. Analysis of sequence variations revealed three types of paralogs MSPA, MSPB and MSPC. Expression of both MSPA and MSPB was detected in testis. In haploid Oreochromis niloticus embryos, each of these types consisted of two closely related paralogs, and asymmetry between MSP copy numbers on the maternal (six copies) and the paternal (three copies) chromosomes was observed. Using this polymorphism we mapped MSPA and MSPC to linkage group 12 of an F2 mapping family derived from a cross between O. niloticus and Oreochromis aureus. Females with high MSP copy number were more frequent by more than twofold than males. Gender-MSPC combinations showed significant deviation from expected Mendelian segregation (P=0.009) suggesting elimination of males with MSPC copies. We discuss different hypotheses to explain this elimination, including possibility for allelic conflict resulted by the hybridization. © 2008 Macmillan Publishers Limited All rights reserved.
Note:
Related Files :
Animals
Female
Genetics
Male
molecular genetics
Oreochromis aureus
Oreochromis niloticus
Tilapia
עוד תגיות
תוכן קשור
More details
DOI :
10.1038/hdy.2008.68
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28568
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
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Scientific Publication
Copy number variation of lipocalin family genes for male-specific proteins in tilapia and its association with gender
101
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Golik, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Lee, B.-Y., Department of Biology, University of Maryland, College Park, MD, United States
Howe, A.E., Department of Biology, University of Maryland, College Park, MD, United States
Kocher, T.D., Department of Biology, University of Maryland, College Park, MD, United States
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
Seroussi, E., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel, Institute of Animal Science, Agricultural Research Organization, Volcani Center, POB6, Beit Dagan 50250, Israel
Copy number variation of lipocalin family genes for male-specific proteins in tilapia and its association with gender
Lipocalins are involved in the binding of small molecules like sex steroids. We show here that the previously reported tilapia male-specific protein (MSP) is a lipocalin encoded by a variety of paralogous and homologous genes in different tilapia species. Exon-intron boundaries of MSP genes were typical of the six-exon genomic structure of lipocalins, and the transcripts were capable of encoding 200 amino-acid polypeptides that consisted of a putative signal peptide and a lipocalin domain. Cysteine residues are conserved in positions analogous to those forming the three disulfide bonds characteristic of the ligand pocket. The calculated molecular mass of the secreted MSP (20.4 kDa) was less than half of that observed, suggesting that it is highly glycosylated like its homologue tributyltin-binding protein. Analysis of sequence variations revealed three types of paralogs MSPA, MSPB and MSPC. Expression of both MSPA and MSPB was detected in testis. In haploid Oreochromis niloticus embryos, each of these types consisted of two closely related paralogs, and asymmetry between MSP copy numbers on the maternal (six copies) and the paternal (three copies) chromosomes was observed. Using this polymorphism we mapped MSPA and MSPC to linkage group 12 of an F2 mapping family derived from a cross between O. niloticus and Oreochromis aureus. Females with high MSP copy number were more frequent by more than twofold than males. Gender-MSPC combinations showed significant deviation from expected Mendelian segregation (P=0.009) suggesting elimination of males with MSPC copies. We discuss different hypotheses to explain this elimination, including possibility for allelic conflict resulted by the hybridization. © 2008 Macmillan Publishers Limited All rights reserved.
Scientific Publication
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