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פותח על ידי קלירמאש פתרונות בע"מ -
The developmental outcomes of P0-mediated ARGONAUTE destabilization in tomato
Year:
2013
Source of publication :
Planta
Authors :
ארזי, צחי
;
.
הנדלמן, ענת
;
.
לוגסי, ניצן
;
.
סתו, רן
;
.
קרבצ'יק, מיכאל
;
.
Volume :
237
Co-Authors:
Hendelman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Kravchik, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Stav, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Zik, M., Department of Life Sciences, Ben Gurion University, 84105 Beer-Sheva, Israel
Lugassi, N., Department of Life Sciences, Ben Gurion University, 84105 Beer-Sheva, Israel
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
363
To page:
377
(
Total pages:
15
)
Abstract:
The plant protein ARGONAUTE1 (AGO1) functions in multiple RNA-silencing pathways, including those of microRNAs, key regulators of growth and development. Genetic analysis of ago1 mutants with informative defects has provided valuable insights into AGO1's biological functions. Tomato encodes two AGO1 homologs (SlAGO1s), but mutants have not been described to date. To analyze SlAGO1s' involvement in development, we confirmed that both undergo decay in the presence of the Polerovirus silencing suppressor P0 and produce a transgenic responder line (OP:P0HA) that, upon transactivation, expresses P0 C-terminally fused to a hemagglutinin (HA) tag (P0HA) and destabilizes SlAGO1s at the site of expression. By crossing OP:P0HA with a battery of driver lines, constitutive as well as organ- and stage-specific SlAGO1 downregulation was induced in the F1 progeny. Activated plants exhibited various developmental phenotypes that partially overlapped with those of Arabidopsis ago1 mutants. Plants that constitutively expressed P0HA had reduced SlAGO1 levels and increased accumulation of miRNA targets, indicating compromised SlAGO1-mediated silencing. Consistent with this, they exhibited pleiotropic morphological defects and their growth was arrested post-germination. Transactivation of P0HA in young leaf and floral organ primordia dramatically modified corresponding organ morphology, including the radialization of leaflets, petals and anthers, suggesting that SlAGO1s' activities are required for normal lateral organ development and polarity. Overall, our results suggest that the OP:P0HA responder line can serve as a valuable tool to suppress SlAGO1 silencing pathways in tomato. The suppression of additional SlAGOs by P0HA and its contribution to the observed phenotypes awaits investigation. © 2012 Springer-Verlag Berlin Heidelberg.
Note:
Related Files :
arabidopsis
Base Sequence
Development
gene expression
Genetics
metabolism
phenotype
ultrastructure
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-012-1778-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28684
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:41
You may also be interested in
Scientific Publication
The developmental outcomes of P0-mediated ARGONAUTE destabilization in tomato
237
Hendelman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Kravchik, M., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Stav, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
Zik, M., Department of Life Sciences, Ben Gurion University, 84105 Beer-Sheva, Israel
Lugassi, N., Department of Life Sciences, Ben Gurion University, 84105 Beer-Sheva, Israel
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, PO BOX 6, 50250 Bet Dagan, Israel
The developmental outcomes of P0-mediated ARGONAUTE destabilization in tomato
The plant protein ARGONAUTE1 (AGO1) functions in multiple RNA-silencing pathways, including those of microRNAs, key regulators of growth and development. Genetic analysis of ago1 mutants with informative defects has provided valuable insights into AGO1's biological functions. Tomato encodes two AGO1 homologs (SlAGO1s), but mutants have not been described to date. To analyze SlAGO1s' involvement in development, we confirmed that both undergo decay in the presence of the Polerovirus silencing suppressor P0 and produce a transgenic responder line (OP:P0HA) that, upon transactivation, expresses P0 C-terminally fused to a hemagglutinin (HA) tag (P0HA) and destabilizes SlAGO1s at the site of expression. By crossing OP:P0HA with a battery of driver lines, constitutive as well as organ- and stage-specific SlAGO1 downregulation was induced in the F1 progeny. Activated plants exhibited various developmental phenotypes that partially overlapped with those of Arabidopsis ago1 mutants. Plants that constitutively expressed P0HA had reduced SlAGO1 levels and increased accumulation of miRNA targets, indicating compromised SlAGO1-mediated silencing. Consistent with this, they exhibited pleiotropic morphological defects and their growth was arrested post-germination. Transactivation of P0HA in young leaf and floral organ primordia dramatically modified corresponding organ morphology, including the radialization of leaflets, petals and anthers, suggesting that SlAGO1s' activities are required for normal lateral organ development and polarity. Overall, our results suggest that the OP:P0HA responder line can serve as a valuable tool to suppress SlAGO1 silencing pathways in tomato. The suppression of additional SlAGOs by P0HA and its contribution to the observed phenotypes awaits investigation. © 2012 Springer-Verlag Berlin Heidelberg.
Scientific Publication
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