אסיף מאגר המחקר החקלאי

Involvement of two different urf-s related mitochondrial sequences in the molecular evolution of the CMS-specific S-Pcf locus in petunia

Year:

1995

Source of publication :

Authors :

Volume :

248

Co-Authors:

Yesodi, V., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Gidoni, D., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Tabib, Y., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Firon, N., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel

Facilitators :

Link :

Abstract:

In petunia, a mitochondrial (mt) locus, S-Pcf, has been found to be strongly associated with cytoplasmic male sterility (CMS). The S-Pcf locus consists of three open reading frames (ORF) that are co-transcribed. The first ORF, Pcf, contains parts of the atp9 and coxII genes and an unidentified reading frame, urf-s. The second and third ORFs contain NADH dehydrogenase subunit 3 (nad3) and ribosomal protein S12 (rps12) sequences, respectively. The nad3 and rps12 sequences included in the S-Pcf locus are identical to the corresponding sequences on the mt genome of fertile petunia. In both CMS and fertile petunia, only a single copy of nad3 and rps12 has been detected on the physical map of the main mt genome. The origin of the urf-s sequence and the molecular events leading to the formation of the chimeric S-Pcf locus are not known. This paper presents evidence indicating that two different mt sequences, related to urf-s and found in fertile petunia lines (orf-h and Rf-1), might have been involved in the molecular evolution of the S-Pcf locus. Southern analysis of mtDNA derived from both fertile and sterile petunia plants suggests that one of these urf-s related sequences (showing 100% homology to urf-s and termed orf-h) is located on a sublimon. An additional, low-homology urf-s related sequence (Rf-1) is shown to be located on the main mt genome 5′ to the nad3 gene. It is, thus, suggested that the sequence of events leading to the generation of the S-Pcf locus might have involved introduction of the orf-h sequence, via homologous recombination, into the main mt genome 5′ to nad3 at the region where the Rf-1 sequence is located. © 1995 Springer-Verlag.

Note:

Related Files :

תוכן קשור

More details

You may also be interested in