Co-Authors:
Wilson, C.L., USDA, Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Wisniewski, M.E., USDA, Agricultural Research Service, Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Droby, S., ARO, The Volcani Center, Bet Dagan, 50250, Israel
Chalutz, E., ARO, The Volcani Center, Bet Dagan, 50250, Israel
Abstract:
Fruit wounds were utilized to screen for potential antagonists to postharvest rot organisms from unidentified microbial populations on fruit surfaces. Washings from apple, oranges, and tomato fruit surfaces were placed in fresh apple fruit wounds which were subsequently challenged with postharvest rot pathogens. After 10 days, those wounds not showing rot development were scraped with a sterile needle. Dislodged material was diluted in sterile water which was diluted serially and plated on nutrient agar. Individual isolations were made from frequently occurring colonies and tested for antagonistic activity against postharvest pathogens. Utilizing these procedures, six isolates were identified which were effective antagonists against Botrytis and Penicillium rots of apple. Three of the isolates were identified as Candida sake (Saito & Ota) Van Uden & Buckley and one as Candida tenuis Diddens & Lodder none of which has been described previously as a biocontrol agent. © 1993.