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פותח על ידי קלירמאש פתרונות בע"מ -
Development of Colletotrichum gloeosporioides restriction enzyme-mediated integration mutants as biocontrol agents against anthracnose disease in avocado fruits
Year:
2001
Source of publication :
Phytopathology
Authors :
זו, ר'
;
.
יעקובי, ניר
;
.
פרוסקי, דב
;
.
קובילר, אילנה
;
.
Volume :
91
Co-Authors:

Yakoby, N., The Hebrew University; Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Zhou, R., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kobiler, I., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., The Hebrew University.
Prusky, D., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel

Facilitators :
From page:
143
To page:
148
(
Total pages:
6
)
Abstract:
Reduced-pathogenicity mutants of the avocado fruit pathogen Colletotrichum gloeosporioides isolate Cg-14 (teleomorph: Glomerella cingulata) were generated by insertional mutagenesis by restriction enzyme-mediated integration (REMI) transformation. Following seven transformations, 3,500 hygromycin-resistant isolates were subjected to a virulence assay by inoculation on mesocarp and pericarp of cv. Fuerte avocado fruits. Fourteen isolates showed a reduced degree of virulence relative compared with wild-type Cg-14. Two isolates, Cg-M-142 and Cg-M-1150, were further characterized. Cg-M-142 produced appressoria on avocado pericarp similar to Cg-14, but caused reduced symptom development on the fruit's pericarp and mesocarp. Isolate Cg-M-1150 did not produce appressoria; it caused much reduced maceration on the mesocarp and no symptoms on the pericarp. Southern blot analysis of Cg-M-142 and Cg-M-1150 showed REMI at different XbaI sites of the fungal genome. Pre-inoculation of avocado fruit with Cg-M-142 delayed symptom development by the wild-type isolate. Induced resistance was accompanied by an increase in the levels of preformed antifungal diene, from 760 to 1,200 μg/g fresh weight 9 days after inoculation, whereas pre-inoculation with Cg-M-1150 did not affect the level of antifungal diene, nor did it delay the appearance of decay symptoms. The results presented here show that reduced-pathogenicity isolates can be used for the biological control of anthracnose caused by C. gloeosporioides attack.
Note:
Related Files :
Avocado
Colletotrichum
fungi
genetic transformation
Inoculation
Persea americana
Plant Disease
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/PHYTO.2001.91.2.143
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28826
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
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Scientific Publication
Development of Colletotrichum gloeosporioides restriction enzyme-mediated integration mutants as biocontrol agents against anthracnose disease in avocado fruits
91

Yakoby, N., The Hebrew University; Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Zhou, R., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kobiler, I., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Dinoor, A., The Hebrew University.
Prusky, D., Dept. Postharv. Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel

Development of Colletotrichum gloeosporioides restriction enzyme-mediated integration mutants as biocontrol agents against anthracnose disease in avocado fruits
Reduced-pathogenicity mutants of the avocado fruit pathogen Colletotrichum gloeosporioides isolate Cg-14 (teleomorph: Glomerella cingulata) were generated by insertional mutagenesis by restriction enzyme-mediated integration (REMI) transformation. Following seven transformations, 3,500 hygromycin-resistant isolates were subjected to a virulence assay by inoculation on mesocarp and pericarp of cv. Fuerte avocado fruits. Fourteen isolates showed a reduced degree of virulence relative compared with wild-type Cg-14. Two isolates, Cg-M-142 and Cg-M-1150, were further characterized. Cg-M-142 produced appressoria on avocado pericarp similar to Cg-14, but caused reduced symptom development on the fruit's pericarp and mesocarp. Isolate Cg-M-1150 did not produce appressoria; it caused much reduced maceration on the mesocarp and no symptoms on the pericarp. Southern blot analysis of Cg-M-142 and Cg-M-1150 showed REMI at different XbaI sites of the fungal genome. Pre-inoculation of avocado fruit with Cg-M-142 delayed symptom development by the wild-type isolate. Induced resistance was accompanied by an increase in the levels of preformed antifungal diene, from 760 to 1,200 μg/g fresh weight 9 days after inoculation, whereas pre-inoculation with Cg-M-1150 did not affect the level of antifungal diene, nor did it delay the appearance of decay symptoms. The results presented here show that reduced-pathogenicity isolates can be used for the biological control of anthracnose caused by C. gloeosporioides attack.
Scientific Publication
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