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Cell cultures vs. whole plants for measuring phytotoxicity I. The establishment and growth of callus and suspension cultures; definition of factors affecting toxicity on calli
Year:
1977
Source of publication :
Plant and Cell Physiology
Authors :
זילכה, שמואל
;
.
Volume :
18
Co-Authors:
Zilkah, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
641
To page:
655
(
Total pages:
15
)
Abstract:
Callus tissue has been isolated from 11 species, of 8 which are 'weeds' from which callus isolation has not been previously described. Continuous suspension cultures of four species have been started. Growth conditions were optimized for high yield. Logarithmic phase and stationary phase cells react differently to some inhibitors. The larger the inoculum size, the greater the concentration of inhibitor that must be used to achieve equal inhibition. Hormonal constitution of the medium affects inhibition by 'auxin' type herbicides. Growth kinetics, delayed response and recovery must be considered in assessing phytotoxicity. Highly uniform and controlled procedures are required for interspecific comparisons of many compounds. © 1977 Oxford University Press.
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Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28847
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
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Scientific Publication
Cell cultures vs. whole plants for measuring phytotoxicity I. The establishment and growth of callus and suspension cultures; definition of factors affecting toxicity on calli
18
Zilkah, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Cell cultures vs. whole plants for measuring phytotoxicity I. The establishment and growth of callus and suspension cultures; definition of factors affecting toxicity on calli
Callus tissue has been isolated from 11 species, of 8 which are 'weeds' from which callus isolation has not been previously described. Continuous suspension cultures of four species have been started. Growth conditions were optimized for high yield. Logarithmic phase and stationary phase cells react differently to some inhibitors. The larger the inoculum size, the greater the concentration of inhibitor that must be used to achieve equal inhibition. Hormonal constitution of the medium affects inhibition by 'auxin' type herbicides. Growth kinetics, delayed response and recovery must be considered in assessing phytotoxicity. Highly uniform and controlled procedures are required for interspecific comparisons of many compounds. © 1977 Oxford University Press.
Scientific Publication
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