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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Mediterranean fruit fly as a potential vector of bacterial pathogens
Year:
2005
Authors :
בר-יוסף, משה
;
.
נמני-לביא, אסתר
;
.
נסטל, דוד
;
.
סלע, שלמה
;
.
פינטו, רבקה
;
.
Volume :
71
Co-Authors:
Sela, S., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Beth-Dagan, Israel, Department of Food Sciences, ARO, Volcani Center, Beth-Dagan 50250, Israel
Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Pinto, R., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Beth-Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Bar-Joseph, M., Department of Virology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Facilitators :
From page:
4052
To page:
4056
(
Total pages:
5
)
Abstract:
The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop eggs. After reaching sexual maturity and copulating, female flies lay eggs in fruit by puncturing the skin with their ovipositors and injecting batches of eggs into the wounds. In view of the increase in food-borne illnesses associated with consumption of fresh produce and unpasteurized fruit juices, we investigated the potential of Mediterranean fruit fly to serve as a vector for transmission of human pathogens to fruits. Addition of green fluorescent protein (GFP)-tagged Escherichia coli to a Mediterranean fruit fly feeding solution resulted in a dose-dependent increase in the fly's bacterial load. Flies exposed to fecal material enriched with GFP-tagged E. coli were similarly contaminated and were capable of transmitting E. coli to intact apples in a cage model system. Washing contaminated apples with tap water did not eliminate the E. coli. Flies inoculated with E. coli harbored the bacteria for up to 7 days following contamination. Fluorescence microscopy demonstrated that the majority of fluorescent bacteria were confined along the pseudotrachea in the labelum edge of the fly proboscis. Wild flies captured at various geographic locations were found to carry coliforms, and in some cases presumptive identification of E. coli was made. These findings support the hypothesis that the common Mediterranean fruit fly is a potential vector of human pathogens to fruits. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Note:
Related Files :
Animals
bacteria
Ceratitis capitata
coliform bacterium
Food Contamination
Fruits
insects
proteins
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.71.7.4052-4056.2005
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28860
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
You may also be interested in
Scientific Publication
Mediterranean fruit fly as a potential vector of bacterial pathogens
71
Sela, S., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Beth-Dagan, Israel, Department of Food Sciences, ARO, Volcani Center, Beth-Dagan 50250, Israel
Nestel, D., Department of Entomology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Pinto, R., Department of Food Sciences, Institute for Technology and Storage of Fresh Produce, Volcani Center, Beth-Dagan, Israel
Nemny-Lavy, E., Department of Entomology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Bar-Joseph, M., Department of Virology, Institute of Plant Protection, Volcani Center, Beth-Dagan, Israel
Mediterranean fruit fly as a potential vector of bacterial pathogens
The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop eggs. After reaching sexual maturity and copulating, female flies lay eggs in fruit by puncturing the skin with their ovipositors and injecting batches of eggs into the wounds. In view of the increase in food-borne illnesses associated with consumption of fresh produce and unpasteurized fruit juices, we investigated the potential of Mediterranean fruit fly to serve as a vector for transmission of human pathogens to fruits. Addition of green fluorescent protein (GFP)-tagged Escherichia coli to a Mediterranean fruit fly feeding solution resulted in a dose-dependent increase in the fly's bacterial load. Flies exposed to fecal material enriched with GFP-tagged E. coli were similarly contaminated and were capable of transmitting E. coli to intact apples in a cage model system. Washing contaminated apples with tap water did not eliminate the E. coli. Flies inoculated with E. coli harbored the bacteria for up to 7 days following contamination. Fluorescence microscopy demonstrated that the majority of fluorescent bacteria were confined along the pseudotrachea in the labelum edge of the fly proboscis. Wild flies captured at various geographic locations were found to carry coliforms, and in some cases presumptive identification of E. coli was made. These findings support the hypothesis that the common Mediterranean fruit fly is a potential vector of human pathogens to fruits. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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