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פותח על ידי קלירמאש פתרונות בע"מ -
Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple
Year:
2003
Source of publication :
Plant Science
Authors :
פישר, ארווין
;
.
פליישמן, משה
;
.
Volume :
165
Co-Authors:
Yancheva, S.D., Plant Biotechnology Laboratory, Agricultural University, 12 Mendeleev Street, 4000 Plovdiv, Bulgaria
Golubowicz, S., Department of Fruit Trees, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Fisher, E., Inst. of Soil Water/Environ. Sci., ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Lev-Yadun, S., Department of Biology, Fac. of Sci. and Science Education, University of Haifa-Oranim, Tivon 36006, Israel
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
299
To page:
309
(
Total pages:
11
)
Abstract:
The effects of auxin and cytokinin on the regulation of de novo apple (cv. Topred) shoot development have been studied through shoot induction, differentiation and development. Leaves were cultured in vitro with Thidiazuron (TDZ) in combination with various auxins. Histological observations showed that within 3 days TDZ with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced more cell divisions than TDZ with indole-3-butyric acid (IBA). One day of exposure to either TDZ + IBA or TDZ + 2,4-D, followed by culture on hormone free medium, initiated cell determination towards shoot regeneration but with only low rates of shoot formation (5-6%). Extension of the culture with TDZ+IBA to 6 days, increased shoot regeneration to approximately 30% and treatment with TDZ+IBA for the whole experimental period (35 days) increased it to 72%. However, a 2-day culture with TDZ+ 2,4-D, followed by TDZ + IBA for 33 days increased regeneration to approximately 80%. TDZ with IBA or with IAA determined cell fate to shoot development, whereas TDZ+2,4-D application for more than 6 days led to the decline of shoot development. The first 6 days of the process form the 'window' of determination of cell fate. We conclude that in apple, the type of auxin, the timing of its application and the length of explant exposure to the specific auxin are critical for the activation and progression of the developmental program. © 2003 Elsevier Science Ireland Ltd. All rights reserved.
Note:
Related Files :
2,4-D
acetic acid
Apple
Cell divisions
Differentiation
IBA
Malus x domestica
organogenesis
Plant cell culture
TDZ
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0168-9452(03)00084-0
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28884
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
Scientific Publication
Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple
165
Yancheva, S.D., Plant Biotechnology Laboratory, Agricultural University, 12 Mendeleev Street, 4000 Plovdiv, Bulgaria
Golubowicz, S., Department of Fruit Trees, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Fisher, E., Inst. of Soil Water/Environ. Sci., ARO, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Lev-Yadun, S., Department of Biology, Fac. of Sci. and Science Education, University of Haifa-Oranim, Tivon 36006, Israel
Flaishman, M.A., Department of Fruit Trees, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple
The effects of auxin and cytokinin on the regulation of de novo apple (cv. Topred) shoot development have been studied through shoot induction, differentiation and development. Leaves were cultured in vitro with Thidiazuron (TDZ) in combination with various auxins. Histological observations showed that within 3 days TDZ with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced more cell divisions than TDZ with indole-3-butyric acid (IBA). One day of exposure to either TDZ + IBA or TDZ + 2,4-D, followed by culture on hormone free medium, initiated cell determination towards shoot regeneration but with only low rates of shoot formation (5-6%). Extension of the culture with TDZ+IBA to 6 days, increased shoot regeneration to approximately 30% and treatment with TDZ+IBA for the whole experimental period (35 days) increased it to 72%. However, a 2-day culture with TDZ+ 2,4-D, followed by TDZ + IBA for 33 days increased regeneration to approximately 80%. TDZ with IBA or with IAA determined cell fate to shoot development, whereas TDZ+2,4-D application for more than 6 days led to the decline of shoot development. The first 6 days of the process form the 'window' of determination of cell fate. We conclude that in apple, the type of auxin, the timing of its application and the length of explant exposure to the specific auxin are critical for the activation and progression of the developmental program. © 2003 Elsevier Science Ireland Ltd. All rights reserved.
Scientific Publication
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