נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Differential effect of actinomycin D on amino acid incorporation by microsomal components of the housefly Musca domestica
Year:
1971
Source of publication :
Experimental Cell Research
Authors :
ישעיה, יצחק
;
.
Volume :
69
Co-Authors:
Ishaaya, I., Research Institute, Canada Department of Agriculture, University Sub P.O., London, Ont., Canada
Chefurka, W., Research Institute, Canada Department of Agriculture, University Sub P.O., London, Ont., Canada
Facilitators :
From page:
81
To page:
91
(
Total pages:
11
)
Abstract:
Sucrose density gradient centrifugation was used to fractionate the microsomes from the housefly Musca domestica into two components, the free ribosomes and the membranes. The effect of actinomycin D (AM) on the in vivo incorporation of leucine into these fractions was studied. Actinomycin D rapidly inhibited RNA synthesis which however, did not result in a rapid inhibition of incorporation of leucine into the free ribosomes. The half-decay time of this inhibition was about 5 h suggesting a relatively stable m RNA. Under identical conditions the incorporation of leucine into the proteins of the membranes was stimulated about two-fold within 2 h after treatment. This enhanced rate of incorporation could be maintained for at least 4 h by a subsequent injection of actinomycin otherwise it rapidly fell off to normal levels. Puromycin completely prevented this stimulation by actinomycin. It is suggested that this enhanced incorporation is due to de-repression of membrane activity as a result of preferential inhibition of synthesis of mRNA for the repressor protein. © 1971.
Note:
Related Files :
Amino Acids
Animal
biosynthesis
Carbon
Female
metabolism
proteins
RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0014-4827(71)90313-2
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28888
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
You may also be interested in
Scientific Publication
Differential effect of actinomycin D on amino acid incorporation by microsomal components of the housefly Musca domestica
69
Ishaaya, I., Research Institute, Canada Department of Agriculture, University Sub P.O., London, Ont., Canada
Chefurka, W., Research Institute, Canada Department of Agriculture, University Sub P.O., London, Ont., Canada
Differential effect of actinomycin D on amino acid incorporation by microsomal components of the housefly Musca domestica
Sucrose density gradient centrifugation was used to fractionate the microsomes from the housefly Musca domestica into two components, the free ribosomes and the membranes. The effect of actinomycin D (AM) on the in vivo incorporation of leucine into these fractions was studied. Actinomycin D rapidly inhibited RNA synthesis which however, did not result in a rapid inhibition of incorporation of leucine into the free ribosomes. The half-decay time of this inhibition was about 5 h suggesting a relatively stable m RNA. Under identical conditions the incorporation of leucine into the proteins of the membranes was stimulated about two-fold within 2 h after treatment. This enhanced rate of incorporation could be maintained for at least 4 h by a subsequent injection of actinomycin otherwise it rapidly fell off to normal levels. Puromycin completely prevented this stimulation by actinomycin. It is suggested that this enhanced incorporation is due to de-repression of membrane activity as a result of preferential inhibition of synthesis of mRNA for the repressor protein. © 1971.
Scientific Publication
You may also be interested in