Co-Authors:
Kleitman, F., Dept. of Plant Pathol. and Weed Res., ARO, Volcani Center, Bet Dagan 50250, Israel
Manulis, S., Dept. of Plant Pathol. and Weed Res., ARO, Volcani Center, Bet Dagan 50250, Israel
Kritzman, G., Dept. of Plant Pathol. and Weed Res., ARO, Volcani Center, Bet Dagan 50250, Israel
Oppenheim, D., Extension Service, Min. of Agric. and Rural Development, Bet Dagan 50250, Israel
Zilberstaine, M., Extension Service, Min. of Agric. and Rural Development, Bet Dagan 50250, Israel
Shtienberg, D., Dept. of Plant Pathol. and Weed Res., ARO, Volcani Center, Bet Dagan 50250, Israel
Abstract:
Erwinia amylovora, the causal agent of fire blight, is managed by application of bactericides to protect fruit tree blossoms from infection. Monitoring the response of E. amylovora strains to bactericides is crucial for adequate disease management. The coliform agar medium produced by Merck was recently reported as an effective tool for rapid diagnosis of E. amylovora (RD-medium). The objective of the present study was to examine the possibility of using the RD-medium for in situ determination of the response of E. amylovora strains to oxolinic acid and streptomycin. The phenotypic response of 48 E. amylovora strains isolated in 2002 to both bactericides was determined with the RD-medium and, for comparison, by a routine laboratory test. The results of 45 samples (93.7%) were in agreement with the findings of the routine laboratory test. A χ2 test rejected the null hypothesis that the phenotypic characteristics as determined by the two respective methods differed significantly (P=0.389). The in situ test was implemented on a national scale in 2003 and the results were in agreement with those obtained in laboratory tests, which suggests that this medium can be used in situ for monitoring the appearance of resistance in E. amylovora populations.
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חסימת אנימציה
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