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פותח על ידי קלירמאש פתרונות בע"מ -
FLP/FRT-mediated restoration of normal phenotypes and clonal sectors formation in rolC transgenic tobacco
Year:
2001
Source of publication :
Transgenic Research
Authors :
גדעוני, דוד
;
.
גלבוע, נחמה
;
.
Volume :
10
Co-Authors:
Gidoni, D., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar, M., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
317
To page:
328
(
Total pages:
12
)
Abstract:
Site-specific recombination systems have been shown to excise transgene DNA sequences positioned between their cognate target sites, and thus be used to generate clonal sectors in transgenic plants. Here we characterized clonal sectors derived from genetic reversion of rolC (A. rhizogenes) - Induced vegetative and reproductive phenotypes, mediated by FLP recombinase from S. cerevisiae, in tobacco. The constitutive expression of rolC induces pleiotropic effects including reduced apical dominance and plant height, lanceolate and pale green leaves and small, male-sterile flowers. Two transgenic male-sterile tobacco lines (N. tabacum, Samsun NN) expressing a 35sP-rolC gene construct flanked by two FRT (FLP recombinase target) sites, were cross-pollinated with pollen from a constitutive 35sP-FLP expressing line. Three main phenotypes were generated in result of recombinase-mediated excision of the 35sP-rolC locus in the F1 (FLP × FRT-35sP-rolC-FRT) hybrid progenies: (a) restoration of male fertility, associated with reversion to normal leaf phenotypes prior to flower bud formation, (b) development of normal and fertile lateral shoot sectors on the background of rolC-type plants, (c) restoration of partially fertile flowers, associated with display of peripheral normal leaf sectors surrounding rolC-type inner-leaf tissues, consistent with periclinal chimeras. These results, supported by DNA molecular analysis, indicate that site-specific recombination might be used as a relatively efficient tool for generation of transgenic periclinal chimeric plants.
Note:
Related Files :
gene expression
genetic line
genetic recombination
phenotype
recombinase
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1016603627254
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28937
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
Scientific Publication
FLP/FRT-mediated restoration of normal phenotypes and clonal sectors formation in rolC transgenic tobacco
10
Gidoni, D., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Bar, M., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Department of Plant Genetics, Institute of Field and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
FLP/FRT-mediated restoration of normal phenotypes and clonal sectors formation in rolC transgenic tobacco
Site-specific recombination systems have been shown to excise transgene DNA sequences positioned between their cognate target sites, and thus be used to generate clonal sectors in transgenic plants. Here we characterized clonal sectors derived from genetic reversion of rolC (A. rhizogenes) - Induced vegetative and reproductive phenotypes, mediated by FLP recombinase from S. cerevisiae, in tobacco. The constitutive expression of rolC induces pleiotropic effects including reduced apical dominance and plant height, lanceolate and pale green leaves and small, male-sterile flowers. Two transgenic male-sterile tobacco lines (N. tabacum, Samsun NN) expressing a 35sP-rolC gene construct flanked by two FRT (FLP recombinase target) sites, were cross-pollinated with pollen from a constitutive 35sP-FLP expressing line. Three main phenotypes were generated in result of recombinase-mediated excision of the 35sP-rolC locus in the F1 (FLP × FRT-35sP-rolC-FRT) hybrid progenies: (a) restoration of male fertility, associated with reversion to normal leaf phenotypes prior to flower bud formation, (b) development of normal and fertile lateral shoot sectors on the background of rolC-type plants, (c) restoration of partially fertile flowers, associated with display of peripheral normal leaf sectors surrounding rolC-type inner-leaf tissues, consistent with periclinal chimeras. These results, supported by DNA molecular analysis, indicate that site-specific recombination might be used as a relatively efficient tool for generation of transgenic periclinal chimeric plants.
Scientific Publication
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