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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Citrus fruit flavor and aroma biosynthesis: Isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of the sesquiterpene aroma compound valencene
Year:
2003
Source of publication :
Plant Journal
Authors :
אור, אתי
;
.
איל, יורם
;
.
בר, עינת
;
.
הולנד, דורון
;
.
לביא, אורי
;
.
לוינסון, אפרים
;
.
פרידמן, אהובה
;
.
שליט, משה
;
.
שרון-אסא, ליאת
;
.
Volume :
36
Co-Authors:
Sharon-Asa, L., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Shalit, M., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Frydman, A., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Bar, E., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Holland, D., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Or, E., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Lavi, U., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Lewinsohn, E., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Eyal, Y., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
664
To page:
674
(
Total pages:
11
)
Abstract:
Citrus fruits possess unique aromas rarely found in other fruit species. While fruit flavor is composed of complex combinations of soluble and volatile compounds, several low-abundance sesquiterpenes, such as valencene, nootkatone, alpha-sinensal, and beta-sinensal, stand out in citrus as important flavor and aroma compounds. The profile of terpenoid volatiles in various citrus species and their importance as aroma compounds have been studied in detail, but much is still lacking in our understanding of the physiological, biochemical, and genetic regulation of their production. Here, we report on the isolation, functional expression, and developmental regulation of Cstps1, a sesquiterpene synthase-encoding gene, involved in citrus aroma formation. The recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene syntheses. Within this group, Cstps1 belongs to a subgroup of citrus sesquiterpene syntheses. Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non-climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence for the role of ethylene in the final stages of citrus fruit ripening. Isolation of the gene encoding valencene synthase provides a tool for an in-depth study of the regulation of aroma compound biosynthesis in citrus and for metabolic engineering for fruit flavor characteristics.
Note:
Related Files :
biosynthesis
enzymes
ethylene
Genes
odors
taste
עוד תגיות
תוכן קשור
More details
DOI :
10.1046/j.1365-313X.2003.01910.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28976
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
Scientific Publication
Citrus fruit flavor and aroma biosynthesis: Isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of the sesquiterpene aroma compound valencene
36
Sharon-Asa, L., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Shalit, M., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Frydman, A., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Bar, E., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Holland, D., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Or, E., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Lavi, U., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Lewinsohn, E., Institute of Field Crops, Newe-Ya'ar Research Center ARO, Ramat-Yishay 30095, Israel
Eyal, Y., Institute of Horticulture, Volcani Center ARO, PO Box 6, Bet-Dagan 50250, Israel
Citrus fruit flavor and aroma biosynthesis: Isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of the sesquiterpene aroma compound valencene
Citrus fruits possess unique aromas rarely found in other fruit species. While fruit flavor is composed of complex combinations of soluble and volatile compounds, several low-abundance sesquiterpenes, such as valencene, nootkatone, alpha-sinensal, and beta-sinensal, stand out in citrus as important flavor and aroma compounds. The profile of terpenoid volatiles in various citrus species and their importance as aroma compounds have been studied in detail, but much is still lacking in our understanding of the physiological, biochemical, and genetic regulation of their production. Here, we report on the isolation, functional expression, and developmental regulation of Cstps1, a sesquiterpene synthase-encoding gene, involved in citrus aroma formation. The recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene syntheses. Within this group, Cstps1 belongs to a subgroup of citrus sesquiterpene syntheses. Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non-climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence for the role of ethylene in the final stages of citrus fruit ripening. Isolation of the gene encoding valencene synthase provides a tool for an in-depth study of the regulation of aroma compound biosynthesis in citrus and for metabolic engineering for fruit flavor characteristics.
Scientific Publication
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