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פותח על ידי קלירמאש פתרונות בע"מ -
Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces
Year:
2010
Source of publication :
BMC Microbiology
Authors :
שמש, משה
;
.
Volume :
10
Co-Authors:
Shemesh, M., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, United States
Tam, A., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Aharoni, R., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Steinberg, D., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background. Adhesion and successful colonization of bacteria onto solid surfaces play a key role in biofilm formation. The initial adhesion and the colonization of bacteria may differ between the various types of surfaces found in oral cavity. Therefore, it is conceivable that diverse biofilms are developed on those various surfaces. The aim of the study was to investigate the molecular modifications occurring during in vitro biofilm development of Streptococcus mutans UA159 on several different dental surfaces. Results. Growth analysis of the immobilized bacterial populations generated on the different surfaces shows that the bacteria constructed a more confluent and thick biofilms on a hydroxyapatite surface compared to the other tested surfaces. Using DNA-microarray technology we identified the differentially expressed genes of S. mutans, reflecting the physiological state of biofilms formed on the different biomaterials tested. Eight selected genes were further analyzed by real time RT-PCR. To further determine the impact of the tested material surfaces on the physiology of the bacteria, we tested the secretion of AI-2 signal by S. mutans embedded on those biofilms. Comparative transcriptome analyses indicated on changes in the S. mutans genome in biofilms formed onto different types of surfaces and enabled us to identify genes most differentially expressed on those surfaces. In addition, the levels of autoinducer-2 in biofilms from the various tested surfaces were different. Conclusions. Our results demonstrate that gene expression of S. mutans differs in biofilms formed on tested surfaces, which manifest the physiological state of bacteria influenced by the type of surface material they accumulate onto. Moreover, the stressful circumstances of adjustment to the surface may persist in the bacteria enhancing intercellular signaling and surface dependent biofilm formation. © 2010 Shemesh et al; licensee BioMed Central Ltd.
Note:
Related Files :
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עוד תגיות
תוכן קשור
More details
DOI :
10.1186/1471-2180-10-51
Article number:
51
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
28987
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
You may also be interested in
Scientific Publication
Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces
10
Shemesh, M., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, United States
Tam, A., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Aharoni, R., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Steinberg, D., Biofilm Research Laboratory, Faculty of Dental Medicine, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces
Background. Adhesion and successful colonization of bacteria onto solid surfaces play a key role in biofilm formation. The initial adhesion and the colonization of bacteria may differ between the various types of surfaces found in oral cavity. Therefore, it is conceivable that diverse biofilms are developed on those various surfaces. The aim of the study was to investigate the molecular modifications occurring during in vitro biofilm development of Streptococcus mutans UA159 on several different dental surfaces. Results. Growth analysis of the immobilized bacterial populations generated on the different surfaces shows that the bacteria constructed a more confluent and thick biofilms on a hydroxyapatite surface compared to the other tested surfaces. Using DNA-microarray technology we identified the differentially expressed genes of S. mutans, reflecting the physiological state of biofilms formed on the different biomaterials tested. Eight selected genes were further analyzed by real time RT-PCR. To further determine the impact of the tested material surfaces on the physiology of the bacteria, we tested the secretion of AI-2 signal by S. mutans embedded on those biofilms. Comparative transcriptome analyses indicated on changes in the S. mutans genome in biofilms formed onto different types of surfaces and enabled us to identify genes most differentially expressed on those surfaces. In addition, the levels of autoinducer-2 in biofilms from the various tested surfaces were different. Conclusions. Our results demonstrate that gene expression of S. mutans differs in biofilms formed on tested surfaces, which manifest the physiological state of bacteria influenced by the type of surface material they accumulate onto. Moreover, the stressful circumstances of adjustment to the surface may persist in the bacteria enhancing intercellular signaling and surface dependent biofilm formation. © 2010 Shemesh et al; licensee BioMed Central Ltd.
Scientific Publication
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