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פותח על ידי קלירמאש פתרונות בע"מ -
Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants
Year:
2005
Source of publication :
Journal of Experimental Botany
Authors :
בדני, חנה
;
.
גלילי, שמואל
;
.
Volume :
56
Co-Authors:
Amira, G., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Ifat, M., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Tal, A., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Hana, B., Department of Agronomy, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Shmuel, G., Department of Agronomy, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rachel, A., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel, Tel-Hai Academic College, Upper Galilee 10120, Israel
Facilitators :
From page:
2443
To page:
2452
(
Total pages:
10
)
Abstract:
With the general aim of elevating the content of the essential amino acid methionine in vegetative tissues of plants, alfalfa (Medicago sativa L.) and tobacco plants, as well as BY2 tobacco suspension cells, were transformed with a β-zein::3HA gene under the 35S promoter of cauliflower mosaic virus encoding a rumen-stable methionine-rich storage protein of 15 kDa zein. To examine whether soluble methionine content limited the accumulation of the 15 kDa zein::3HA, methionine was first added to the growth medium of the different transgenic plants and the level of the alien protein was determined. Results demonstrated that the added methionine enhanced the accumulation of the 15 kDa zein::3HA in transgenic alfalfa and tobacco BY2 cells, but not in whole transgenic tobacco plants. Next, the endogenous levels of methionine were elevated in the transgenic tobacco and alfalfa plants by crossing them with plants expressing the Arabidopsis cystathionine γ-synthase (AtCGS) having significantly higher levels of soluble methionine in their leaves. Compared with plants expressing only the 15 kDa zein::3HA, transgenic alfalfa co-expressing both alien genes showed significantly enhanced levels of this protein concurrently with a reduction in the soluble methionine content, thus implying that soluble methionine was incorporated into the 15 kDa zein::3HA. Similar phenomena also occurred in tobacco, but were considerably less pronounced. The results demonstrate that the accumulation of the 15 kDa zein::3HA is regulated in a species-specific manner and that soluble methionine plays a major role in the accumulation of the 15 kDa zein in some plant species but less so in others. © The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
Note:
Related Files :
Amino Acids
arabidopsis
Genetics
Medicago sativa
metabolism
proteins
transgenic plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/eri237
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29054
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
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Scientific Publication
Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants
56
Amira, G., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Ifat, M., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Tal, A., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel
Hana, B., Department of Agronomy, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Shmuel, G., Department of Agronomy, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rachel, A., Plant Science Laboratory, Migal-Galilee Technology Center, PO Box 831, Kiryat Shmona, 11016, Israel, Tel-Hai Academic College, Upper Galilee 10120, Israel
Soluble methionine enhances accumulation of a 15 kDa zein, a methionine-rich storage protein, in transgenic alfalfa but not in transgenic tobacco plants
With the general aim of elevating the content of the essential amino acid methionine in vegetative tissues of plants, alfalfa (Medicago sativa L.) and tobacco plants, as well as BY2 tobacco suspension cells, were transformed with a β-zein::3HA gene under the 35S promoter of cauliflower mosaic virus encoding a rumen-stable methionine-rich storage protein of 15 kDa zein. To examine whether soluble methionine content limited the accumulation of the 15 kDa zein::3HA, methionine was first added to the growth medium of the different transgenic plants and the level of the alien protein was determined. Results demonstrated that the added methionine enhanced the accumulation of the 15 kDa zein::3HA in transgenic alfalfa and tobacco BY2 cells, but not in whole transgenic tobacco plants. Next, the endogenous levels of methionine were elevated in the transgenic tobacco and alfalfa plants by crossing them with plants expressing the Arabidopsis cystathionine γ-synthase (AtCGS) having significantly higher levels of soluble methionine in their leaves. Compared with plants expressing only the 15 kDa zein::3HA, transgenic alfalfa co-expressing both alien genes showed significantly enhanced levels of this protein concurrently with a reduction in the soluble methionine content, thus implying that soluble methionine was incorporated into the 15 kDa zein::3HA. Similar phenomena also occurred in tobacco, but were considerably less pronounced. The results demonstrate that the accumulation of the 15 kDa zein::3HA is regulated in a species-specific manner and that soluble methionine plays a major role in the accumulation of the 15 kDa zein in some plant species but less so in others. © The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
Scientific Publication
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