חיפוש מתקדם
plant biology (source)
Swartzberg, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Dai, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gan, S., Department of Horticulture, Cornell University, Ithaca, NY 14853-5904, United States
Amasino, R., University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Two promoters of senescence-associated Arabidopsis genes, SAG12 and SAG13, were used in tomato plants to express IPT that catalyzes the rate-limiting step in cytokinin biosynthesis. Expression of these heterologous promoters in tomato plants was analyzed using the reporter gene β-glucuronidase. Both promoters are expressed in tomato leaves in a manner similar to their expression in Arabidopsis plants. The SAG12 promoter is very specific to senescing leaves, whereas the SAG13 promoter is expressed in mature leaves prior to the onset of visible senescence and its expression increases in senescing leaves. Expression of both promoters in tomato tissues other than leaves was very low. IPT expressed under the control of S4G12 and SAG13 promoters (P SAG12::IPT and P SAG13::IPT, respectively) resulted in suppression of leaf senescence and advanced flowering, as well as in a slight increase in fruit weight and fruit total soluble solids (TSS). However, expression of P SAG13::IPT also led to stem thickening, short internodal distances and loss of apical dominance. In contrast to the autoregulation of P SAG12::IPT, P SAG13::IPT is expressed at higher levels in mature leaves. This difference is likely due to P SAG13::IPT exhibiting two phases of expression - a senescence-independent expression prior to the onset of senescence that is not subjected to autoregulation by cytokinin, and enhanced expression throughout senescence which is autoregualted by cytokinin. This moderate different autoregulated behavior of P SAG12::IPT and P SAG13::IPT markedly influenced plant development, emphasizing the biological effects of cytokinin in addition to senescence inhibition. © Georg Thieme Verlag KG Stuttgart.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
Effects of cytokinin production under two SAG promoters on senescence and development of tomato plants
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Swartzberg, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Dai, N., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Gan, S., Department of Horticulture, Cornell University, Ithaca, NY 14853-5904, United States
Amasino, R., University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Effects of cytokinin production under two SAG promoters on senescence and development of tomato plants
Two promoters of senescence-associated Arabidopsis genes, SAG12 and SAG13, were used in tomato plants to express IPT that catalyzes the rate-limiting step in cytokinin biosynthesis. Expression of these heterologous promoters in tomato plants was analyzed using the reporter gene β-glucuronidase. Both promoters are expressed in tomato leaves in a manner similar to their expression in Arabidopsis plants. The SAG12 promoter is very specific to senescing leaves, whereas the SAG13 promoter is expressed in mature leaves prior to the onset of visible senescence and its expression increases in senescing leaves. Expression of both promoters in tomato tissues other than leaves was very low. IPT expressed under the control of S4G12 and SAG13 promoters (P SAG12::IPT and P SAG13::IPT, respectively) resulted in suppression of leaf senescence and advanced flowering, as well as in a slight increase in fruit weight and fruit total soluble solids (TSS). However, expression of P SAG13::IPT also led to stem thickening, short internodal distances and loss of apical dominance. In contrast to the autoregulation of P SAG12::IPT, P SAG13::IPT is expressed at higher levels in mature leaves. This difference is likely due to P SAG13::IPT exhibiting two phases of expression - a senescence-independent expression prior to the onset of senescence that is not subjected to autoregulation by cytokinin, and enhanced expression throughout senescence which is autoregualted by cytokinin. This moderate different autoregulated behavior of P SAG12::IPT and P SAG13::IPT markedly influenced plant development, emphasizing the biological effects of cytokinin in addition to senescence inhibition. © Georg Thieme Verlag KG Stuttgart.
Scientific Publication
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