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Journal of General Virology
Peng, Y.-H., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Kadoury, D., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Huet, H., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Wang, Y., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Raccah, B., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Transmission of zucchini yellow mosaic virus (ZYMV) by aphids was examined by introducing mutations within the highly conserved proline-threonine-lysine (PTK) motif of the helper component proteinase (HC-Pro) using a cDNA full-length clone. Replacement of proline by alanine (ATK) in the PTK motif abolished transmission almost completely both from plants and from membranes. Substitution of the basic lysine by glutamic acid (PTE) did not reduce the rate of transmission compared with the wild-type. Replacement of threonine by valine (PVK) or serine (PSK) resulted in a rate of transmission that was lower than that of the wild-type. The rate was lower for PSK than for PVK. Western blot comparison did not permit attribution of HC-Pro functionality in transmission to its level in the host. The HC-Pro of strains that effected transmission (with the wild-type PTK motif, and with the mutated PTE and PVK motifs) could also bind in vitro to virions of ZYMV. HC-Pro with a PSK motif, which was less effective in assisting transmission, could bind only weakly to virions, while HC-Pro of the almost non-transmissible strains (with PAK and ATK motifs) did not bind at all. Interestingly, positive binding was recorded for transmission-defective ZYMV-Ct, which has a PTK motif but has glutamic acid instead of lysine in the lysine-leucine-serine-cysteine (KLSC) motif. These findings support the 'bridge hypothesis', and confirm the binding of the HC-Pro to the virion. The possible role of the PTK and KLSC motifs in binding to the virus and to the mouthparts of the aphid is discussed.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: Effects on aphid transmission and binding to purified virions
79
Peng, Y.-H., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Kadoury, D., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Huet, H., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Wang, Y., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Raccah, B., Department of Virology, Volcani Center, Post Box 6, Bet Dagan 50-250, Israel
Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: Effects on aphid transmission and binding to purified virions
Transmission of zucchini yellow mosaic virus (ZYMV) by aphids was examined by introducing mutations within the highly conserved proline-threonine-lysine (PTK) motif of the helper component proteinase (HC-Pro) using a cDNA full-length clone. Replacement of proline by alanine (ATK) in the PTK motif abolished transmission almost completely both from plants and from membranes. Substitution of the basic lysine by glutamic acid (PTE) did not reduce the rate of transmission compared with the wild-type. Replacement of threonine by valine (PVK) or serine (PSK) resulted in a rate of transmission that was lower than that of the wild-type. The rate was lower for PSK than for PVK. Western blot comparison did not permit attribution of HC-Pro functionality in transmission to its level in the host. The HC-Pro of strains that effected transmission (with the wild-type PTK motif, and with the mutated PTE and PVK motifs) could also bind in vitro to virions of ZYMV. HC-Pro with a PSK motif, which was less effective in assisting transmission, could bind only weakly to virions, while HC-Pro of the almost non-transmissible strains (with PAK and ATK motifs) did not bind at all. Interestingly, positive binding was recorded for transmission-defective ZYMV-Ct, which has a PTK motif but has glutamic acid instead of lysine in the lysine-leucine-serine-cysteine (KLSC) motif. These findings support the 'bridge hypothesis', and confirm the binding of the HC-Pro to the virion. The possible role of the PTK and KLSC motifs in binding to the virus and to the mouthparts of the aphid is discussed.
Scientific Publication
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