נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.
Year:
2004
Source of publication :
Planta
Authors :
נדלר-חסר, טליה
;
.
Volume :
219
Co-Authors:
Nadler-Hassar, T., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Goldshmidt, A., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Rubin, B., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Wolf, S., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Facilitators :
From page:
790
To page:
796
(
Total pages:
7
)
Abstract:
The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host. © Springer-Verlag 2004.
Note:
Related Files :
Cuscuta
Genetics
herbicides
metabolism
parasites
Plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-004-1288-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29205
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
You may also be interested in
Scientific Publication
Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.
219
Nadler-Hassar, T., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Goldshmidt, A., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Rubin, B., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Wolf, S., R.H. Smith Inst. Plant Sci. G., Fac. Agric., Food Environ. Qual. S., Hebrew University of Jerusalem, 76100 Rehovot, Israel
Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.
The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host. © Springer-Verlag 2004.
Scientific Publication
You may also be interested in