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פותח על ידי קלירמאש פתרונות בע"מ -
Adaptation to nicotine in the facultative tobacco-feeding hemipteran Bemisia tabaci
Year:
2014
Source of publication :
Pest Management Science
Authors :
גנאים, מוראד
;
.
קונצדלוב, סבטלנה
;
.
קליאוט, עדי
;
.
Volume :
70
Co-Authors:
Kliot, A., Department of Entomology, Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Department of Entomology, Volcani Center, Bet Dagan, Israel
Ramsey, J.S., Boyce Thompson Institute, Ithaca, NY, United States
Jander, G., Boyce Thompson Institute, Ithaca, NY, United States
Ghanim, M., Department of Entomology, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
1595
To page:
1603
(
Total pages:
9
)
Abstract:
BACKGROUND: Plant defensive metabolites such as nicotine can provide barriers to host-range expansion by generalist herbivores. Nicotine is one of the most abundant and toxic plant secondary metabolites in nature and is defined by high toxicity to plant-feeding insects. There is significant variation in nicotine tolerance among Bemisia tabaci (tobacco whitefly) isolates. Some nicotine-tolerant B. tabaci strains can consume 40-fold higher nicotine levels than susceptible strains, and also show cross-resistance to neonicotinoid insecticides. In this study, biological and molecular assays were used to investigate the responses of B. tabaci strains that differ in their ability to tolerate dietary nicotine. RESULTS: Egg laying and honeydew secretion bioassays as well as gene expression microarrays were used to measure B. tabaci biological parameters and gene transcripts misregulated in response to nicotine in resistant and susceptible strains. The resistant B. tabaci strain laid significantly fewer eggs and excreted more honeydew on a tobacco strain with high levels of nicotine, suggesting a fitness cost effect. The molecular response was drastic in the susceptible strain, while the resistant strain exhibited moderate response. Higher expression of the previously identified CYP6CM1 P450 monooxygenase gene related to the resistance to neonicotinoids, as well as other P450s and metabolic genes, was identified in the resistant and susceptible strains after exposure to nicotine. CONCLUSIONS: Nicotine is a very toxic plant natural compound, and its mode of action resembles that of synthetic neonicotinoids. The biological and molecular responses observed in this study suggest that nicotine may play an important role in providing barriers for host-plant expansion by generalists, and may act as a natural factor that contributes to the development of insect populations resistant to synthetic pesticides. © 2014 Society of Chemical Industry.
Note:
Related Files :
Animal
Animals
Bemisia tabaci
gene expression
Genetics
insecticides
nicotine
Parasitology
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/ps.3739
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29221
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
Scientific Publication
Adaptation to nicotine in the facultative tobacco-feeding hemipteran Bemisia tabaci
70
Kliot, A., Department of Entomology, Volcani Center, Bet Dagan, Israel
Kontsedalov, S., Department of Entomology, Volcani Center, Bet Dagan, Israel
Ramsey, J.S., Boyce Thompson Institute, Ithaca, NY, United States
Jander, G., Boyce Thompson Institute, Ithaca, NY, United States
Ghanim, M., Department of Entomology, Volcani Center, Bet Dagan, Israel
Adaptation to nicotine in the facultative tobacco-feeding hemipteran Bemisia tabaci
BACKGROUND: Plant defensive metabolites such as nicotine can provide barriers to host-range expansion by generalist herbivores. Nicotine is one of the most abundant and toxic plant secondary metabolites in nature and is defined by high toxicity to plant-feeding insects. There is significant variation in nicotine tolerance among Bemisia tabaci (tobacco whitefly) isolates. Some nicotine-tolerant B. tabaci strains can consume 40-fold higher nicotine levels than susceptible strains, and also show cross-resistance to neonicotinoid insecticides. In this study, biological and molecular assays were used to investigate the responses of B. tabaci strains that differ in their ability to tolerate dietary nicotine. RESULTS: Egg laying and honeydew secretion bioassays as well as gene expression microarrays were used to measure B. tabaci biological parameters and gene transcripts misregulated in response to nicotine in resistant and susceptible strains. The resistant B. tabaci strain laid significantly fewer eggs and excreted more honeydew on a tobacco strain with high levels of nicotine, suggesting a fitness cost effect. The molecular response was drastic in the susceptible strain, while the resistant strain exhibited moderate response. Higher expression of the previously identified CYP6CM1 P450 monooxygenase gene related to the resistance to neonicotinoids, as well as other P450s and metabolic genes, was identified in the resistant and susceptible strains after exposure to nicotine. CONCLUSIONS: Nicotine is a very toxic plant natural compound, and its mode of action resembles that of synthetic neonicotinoids. The biological and molecular responses observed in this study suggest that nicotine may play an important role in providing barriers for host-plant expansion by generalists, and may act as a natural factor that contributes to the development of insect populations resistant to synthetic pesticides. © 2014 Society of Chemical Industry.
Scientific Publication
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