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פותח על ידי קלירמאש פתרונות בע"מ -
Lipopolysaccharide challenge of the mammary gland in bovine induced a transient glandular shift to anaerobic metabolism
Year:
2011
Source of publication :
Journal of Dairy Science
Authors :
סילניקוב, ניסים
;
.
ראוך-כהן, עדי
;
.
שפירו, פירה
;
.
Volume :
94
Co-Authors:
Silanikove, N., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Rauch-Cohen, A., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Shapiro, F., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Blum, S., Kimaron Veterinary Institute, National Mastitis Center, POB 12, Bet Dagan 50250, Israel
Arieli, A., Department of Animal Science, Faculty of Agricultural, Food and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76-100, Israel
Leitner, G., Kimaron Veterinary Institute, National Mastitis Center, POB 12, Bet Dagan 50250, Israel
Facilitators :
From page:
4468
To page:
4475
(
Total pages:
8
)
Abstract:
Support of milk production in modern dairy cows demands a large proportion of its own metabolic resources, such as glucose, which might be required under stressful situations. The aim of the experiment was to test the hypothesis that acute immune stress shifts oxidative metabolism to glycolysis. Two mammary quarters in 6 Holstein cows were infused with lipopolysaccharide (LPS), whereas the 2 counter quarters served as controls to the treatment. An additional 6 cows were infused with saline and served as running controls. The LPS challenge induced dramatic transient increases in milk lactate (75-fold) and malate (11-fold) concentrations (both markers of glycolysis) at 24. h posttreatment. No significant changes in lactate and malate concentrations were recorded in control quarters and control animals, indicating that the effect of LPS was restricted to the treated gland. The LPS challenge induced a dramatic transient decrease in milk yield, and lactose and citrate (a marker of mitochondrial metabolism) secretion at 24. h posttreatment. The kinetics were inversely proportional to those of lactate and malate concentrations. Thus, our data suggest that LPS challenge induces acute conversion of epithelial cell metabolism from principally mitochondrial-oxidative to principally cytosolic (glycolytic), which allows the diversion of metabolic resources normally used to synthesize milk to support the immune system. An in vitro bacterial growth test showed that concentrations of lactate, malate, and lactose equivalent to those found in the in vivo experiment delayed and reduced the growth of a pathogenic Escherichia coli strain, suggesting that they play a role in diminution of bacterial multiplication in the mammary gland. © 2011 American Dairy Science Association.
Note:
Related Files :
Animal
Animals
cattle
Female
Growth, Development and Aging
lactation
metabolism
milk
עוד תגיות
תוכן קשור
More details
DOI :
10.3168/jds.2010-4092
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29291
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
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Scientific Publication
Lipopolysaccharide challenge of the mammary gland in bovine induced a transient glandular shift to anaerobic metabolism
94
Silanikove, N., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Rauch-Cohen, A., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Shapiro, F., Biology of Lactation Laboratory, Institute of Animal Science, The Volcani Center, POB 6, Bet Dagan 50250, Israel
Blum, S., Kimaron Veterinary Institute, National Mastitis Center, POB 12, Bet Dagan 50250, Israel
Arieli, A., Department of Animal Science, Faculty of Agricultural, Food and Environmental Sciences, Hebrew University of Jerusalem, Rehovot 76-100, Israel
Leitner, G., Kimaron Veterinary Institute, National Mastitis Center, POB 12, Bet Dagan 50250, Israel
Lipopolysaccharide challenge of the mammary gland in bovine induced a transient glandular shift to anaerobic metabolism
Support of milk production in modern dairy cows demands a large proportion of its own metabolic resources, such as glucose, which might be required under stressful situations. The aim of the experiment was to test the hypothesis that acute immune stress shifts oxidative metabolism to glycolysis. Two mammary quarters in 6 Holstein cows were infused with lipopolysaccharide (LPS), whereas the 2 counter quarters served as controls to the treatment. An additional 6 cows were infused with saline and served as running controls. The LPS challenge induced dramatic transient increases in milk lactate (75-fold) and malate (11-fold) concentrations (both markers of glycolysis) at 24. h posttreatment. No significant changes in lactate and malate concentrations were recorded in control quarters and control animals, indicating that the effect of LPS was restricted to the treated gland. The LPS challenge induced a dramatic transient decrease in milk yield, and lactose and citrate (a marker of mitochondrial metabolism) secretion at 24. h posttreatment. The kinetics were inversely proportional to those of lactate and malate concentrations. Thus, our data suggest that LPS challenge induces acute conversion of epithelial cell metabolism from principally mitochondrial-oxidative to principally cytosolic (glycolytic), which allows the diversion of metabolic resources normally used to synthesize milk to support the immune system. An in vitro bacterial growth test showed that concentrations of lactate, malate, and lactose equivalent to those found in the in vivo experiment delayed and reduced the growth of a pathogenic Escherichia coli strain, suggesting that they play a role in diminution of bacterial multiplication in the mammary gland. © 2011 American Dairy Science Association.
Scientific Publication
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