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פותח על ידי קלירמאש פתרונות בע"מ -
The full-sib intercross line (FSIL): A QTL mapping design for outcrossing species
Year:
1999
Source of publication :
Genetical Research
Authors :
גניזי, אברהם
;
.
Volume :
73
Co-Authors:
Song, J.Z., Department of Genetics, Silberman Life Sciences Institute, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Soller, M., Department of Genetics, Silberman Life Sciences Institute, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Genizi, A., Department of Statistics, Agricultural Research Organization, POB 6, Bet Dagan 50250, Israel
Facilitators :
From page:
61
To page:
73
(
Total pages:
13
)
Abstract:
A full-sib intercross line (FSIL) is constructed in an outcrossing species by mating two parents and intercrossing their progeny to form a large intercross line. For given statistical power, a FSIL design requires only slightly more individuals than an F2 design derived from inbred line cross, but 6- to 10-fold fewer than a half-sib or full-sib design. Due to population-wide linkage disequilibrium, a FSIL is amenable to analysis by selective DNA pooling. In addition, a FSIL is maintained by continued intercrossing so that DNA samples and phenotypic information are accumulated across generations. Continued intercrossing also leads to map expansion and thus to increased mapping accuracy in the later generations. A FSIL can thus provide a bridge to positional cloning of quantitative trait loci (QTL) and marker-assisted selection in outcrossers; and is particularly effective in exploiting the QTL mapping potential of crosses between selection lines or phenotypically differentiated populations that differ in frequency, but are not at fixation, for alternative QTL alleles. In the course of the power analyses, it is shown that for F2 and FSIL designs, power is a function of Nd2 alone, where N is the total size of the mapping population and d is the standardized gene effect; while for half-sib and full-sib populations, power is a function of Nd2 and of the number of families included in the mapping population. This provides a convenient means of estimating power for a wide variety of mapping designs.
Note:
Related Files :
allele
article
gene linkage disequilibrium
gene locus
gene mapping
inbreeding
parent
progeny
quantitative trait
עוד תגיות
תוכן קשור
More details
DOI :
10.1017/S0016672398003486
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29311
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
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Scientific Publication
The full-sib intercross line (FSIL): A QTL mapping design for outcrossing species
73
Song, J.Z., Department of Genetics, Silberman Life Sciences Institute, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Soller, M., Department of Genetics, Silberman Life Sciences Institute, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
Genizi, A., Department of Statistics, Agricultural Research Organization, POB 6, Bet Dagan 50250, Israel
The full-sib intercross line (FSIL): A QTL mapping design for outcrossing species
A full-sib intercross line (FSIL) is constructed in an outcrossing species by mating two parents and intercrossing their progeny to form a large intercross line. For given statistical power, a FSIL design requires only slightly more individuals than an F2 design derived from inbred line cross, but 6- to 10-fold fewer than a half-sib or full-sib design. Due to population-wide linkage disequilibrium, a FSIL is amenable to analysis by selective DNA pooling. In addition, a FSIL is maintained by continued intercrossing so that DNA samples and phenotypic information are accumulated across generations. Continued intercrossing also leads to map expansion and thus to increased mapping accuracy in the later generations. A FSIL can thus provide a bridge to positional cloning of quantitative trait loci (QTL) and marker-assisted selection in outcrossers; and is particularly effective in exploiting the QTL mapping potential of crosses between selection lines or phenotypically differentiated populations that differ in frequency, but are not at fixation, for alternative QTL alleles. In the course of the power analyses, it is shown that for F2 and FSIL designs, power is a function of Nd2 alone, where N is the total size of the mapping population and d is the standardized gene effect; while for half-sib and full-sib populations, power is a function of Nd2 and of the number of families included in the mapping population. This provides a convenient means of estimating power for a wide variety of mapping designs.
Scientific Publication
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