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פותח על ידי קלירמאש פתרונות בע"מ -
Tiron as a substrate for horseradish peroxidase
Year:
1989
Source of publication :
Phytochemistry
Authors :
כהן, ורדה
;
.
Volume :
28
Co-Authors:
Kahn, V., Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
41
To page:
44
(
Total pages:
4
)
Abstract:
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid in the presence of hydrogen peroxide, can serve as a hydrogen donor for horseradish peroxidase. The product(s) formed is yellow and is characterized by a peak at 435 nm. The relationship between, and effect of, various concentrations of horseradish peroxidase, hydrogen peroxide and Tiron on the rate of oxidation of Tiron to the yellow product(s) are described. Partial purification of the yellow product(s) was achieved by chromatography on Sephadex G-10 Sephadex G-25 and Sephadex G-50. The data suggest that the yellow product(s) formed is a low - Mr polymer of Tiron-o-quinone. © 1989.
Note:
Related Files :
horseradish peroxidase
Tiron
yellow product.
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0031-9422(89)85003-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29351
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:46
Scientific Publication
Tiron as a substrate for horseradish peroxidase
28
Kahn, V., Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Tiron as a substrate for horseradish peroxidase
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid in the presence of hydrogen peroxide, can serve as a hydrogen donor for horseradish peroxidase. The product(s) formed is yellow and is characterized by a peak at 435 nm. The relationship between, and effect of, various concentrations of horseradish peroxidase, hydrogen peroxide and Tiron on the rate of oxidation of Tiron to the yellow product(s) are described. Partial purification of the yellow product(s) was achieved by chromatography on Sephadex G-10 Sephadex G-25 and Sephadex G-50. The data suggest that the yellow product(s) formed is a low - Mr polymer of Tiron-o-quinone. © 1989.
Scientific Publication
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