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Phytochemistry
Kahn, V., Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid in the presence of hydrogen peroxide, can serve as a hydrogen donor for horseradish peroxidase. The product(s) formed is yellow and is characterized by a peak at 435 nm. The relationship between, and effect of, various concentrations of horseradish peroxidase, hydrogen peroxide and Tiron on the rate of oxidation of Tiron to the yellow product(s) are described. Partial purification of the yellow product(s) was achieved by chromatography on Sephadex G-10 Sephadex G-25 and Sephadex G-50. The data suggest that the yellow product(s) formed is a low - Mr polymer of Tiron-o-quinone. © 1989.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Tiron as a substrate for horseradish peroxidase
28
Kahn, V., Department of Food Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Tiron as a substrate for horseradish peroxidase
Tiron (4,5-dihydroxy-1,3-benzene disulphonic acid in the presence of hydrogen peroxide, can serve as a hydrogen donor for horseradish peroxidase. The product(s) formed is yellow and is characterized by a peak at 435 nm. The relationship between, and effect of, various concentrations of horseradish peroxidase, hydrogen peroxide and Tiron on the rate of oxidation of Tiron to the yellow product(s) are described. Partial purification of the yellow product(s) was achieved by chromatography on Sephadex G-10 Sephadex G-25 and Sephadex G-50. The data suggest that the yellow product(s) formed is a low - Mr polymer of Tiron-o-quinone. © 1989.
Scientific Publication
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