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פותח על ידי קלירמאש פתרונות בע"מ -
Local modulation of host pH by Colletotrichum species as a mechanism to increase virulence
Year:
2001
Authors :
פרוסקי, דב
;
.
Volume :
14
Co-Authors:
Prusky, D., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
McEvoy, J.L., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Leverentz, B., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Conway, W.S., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Facilitators :
From page:
1105
To page:
1113
(
Total pages:
9
)
Abstract:
The phytopathogenic fungus Colletotrichum gloeosporioides produces one pectate lyase (PL) that is a key virulence factor in disease development. During growth of C. gloeosporioides, Colletotrichum acutatum, and Colletotrichum coccodes in acidified yeast extract medium, the fungus secreted ammonia and increased the medium pH. Ammonia accumulation and the consequent pH change increased as a function of initial pH and buffer capacity of the medium. PL secretion by C. gloeosporioides correspondingly increased as the pH of the medium increased. The C. gloeosporioides pelB gene-disrupted mutant was able to increase ammonia accumulation and pH of the media similarly to the wild-type isolate. C. gloeosporioides in avocado, C. coccodes in tomato, and C. acutatum in apple showed ammonia accumulation in the infected area where pH increased to 7.5 to 8 and PL activity is optima. In nonhost interactions where C. gloeosporioides was inoculated in apples, the addition of ammonia-releasing compounds significantly enhanced pathogenicity to levels similar to those caused by the compatible C. acutatum-apple interaction. The results therefore suggest the importance of ammonia secretion as a virulence factor, enhancing environmental pH and pathogenicity of the Colletotrichum species.
Note:
Related Files :
Colletotrichum
fungi
Genes, Fungal
mutation
Persea americana
pH
Plant Disease
Plant Diseases
virulence
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29394
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:46
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Scientific Publication
Local modulation of host pH by Colletotrichum species as a mechanism to increase virulence
14
Prusky, D., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
McEvoy, J.L., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Leverentz, B., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Conway, W.S., Produce Quality Laboratory, H.A.W. Beltsville Agric. Res. Ctr., U.S. Department of Agriculture, Beltsville, MD 20705, United States
Local modulation of host pH by Colletotrichum species as a mechanism to increase virulence
The phytopathogenic fungus Colletotrichum gloeosporioides produces one pectate lyase (PL) that is a key virulence factor in disease development. During growth of C. gloeosporioides, Colletotrichum acutatum, and Colletotrichum coccodes in acidified yeast extract medium, the fungus secreted ammonia and increased the medium pH. Ammonia accumulation and the consequent pH change increased as a function of initial pH and buffer capacity of the medium. PL secretion by C. gloeosporioides correspondingly increased as the pH of the medium increased. The C. gloeosporioides pelB gene-disrupted mutant was able to increase ammonia accumulation and pH of the media similarly to the wild-type isolate. C. gloeosporioides in avocado, C. coccodes in tomato, and C. acutatum in apple showed ammonia accumulation in the infected area where pH increased to 7.5 to 8 and PL activity is optima. In nonhost interactions where C. gloeosporioides was inoculated in apples, the addition of ammonia-releasing compounds significantly enhanced pathogenicity to levels similar to those caused by the compatible C. acutatum-apple interaction. The results therefore suggest the importance of ammonia secretion as a virulence factor, enhancing environmental pH and pathogenicity of the Colletotrichum species.
Scientific Publication
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