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Methods in Molecular Biology

Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
 

Flowering shoots offer a very convenient and excellent model system for in-depth study of shoot gravitropism in regular stems rather than in special aboveground organs, showing how plants cope with the force of gravity on Earth and change in orientation. Regarding the emerging notion that roots and shoots execute their gravitropic bending by different mechanisms, the use of flowering shoots offers additional confirmation for the suggested shoot-sensing mechanisms initially found in Arabidopsis. As a part of confirming this mechanism, studying this unique model system also enabled elucidation of the sequence of events operating in gravity signalling in shoots. Hence, using the system of flowering shoots provided an additional dimension to our understanding of shoot gravitropism and its hormonal regulation, which has been less advanced than root gravitropism. This is particularly important since the term "shoots" includes various aboveground organs. Hence, unlike other aboveground organs such as pulvini, the asymmetric growth in response to change in shoot orientation is accompanied in cut ornamental spikes by a continuous growth process. This chapter provides an overview of the basic methods, specifically developed or adapted from other graviresponding systems, for determining the main components which play a key role in gravistimulation signalling in flowering shoots. © Springer Science+Business Media New York 2015. All right reserved.
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Flowering shoots of ornamental crops as a model to study cellular and molecular aspects of plant gravitropism
1309

Philosoph-Hadas, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
Meir, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel
 

Flowering shoots of ornamental crops as a model to study cellular and molecular aspects of plant gravitropism
Flowering shoots offer a very convenient and excellent model system for in-depth study of shoot gravitropism in regular stems rather than in special aboveground organs, showing how plants cope with the force of gravity on Earth and change in orientation. Regarding the emerging notion that roots and shoots execute their gravitropic bending by different mechanisms, the use of flowering shoots offers additional confirmation for the suggested shoot-sensing mechanisms initially found in Arabidopsis. As a part of confirming this mechanism, studying this unique model system also enabled elucidation of the sequence of events operating in gravity signalling in shoots. Hence, using the system of flowering shoots provided an additional dimension to our understanding of shoot gravitropism and its hormonal regulation, which has been less advanced than root gravitropism. This is particularly important since the term "shoots" includes various aboveground organs. Hence, unlike other aboveground organs such as pulvini, the asymmetric growth in response to change in shoot orientation is accompanied in cut ornamental spikes by a continuous growth process. This chapter provides an overview of the basic methods, specifically developed or adapted from other graviresponding systems, for determining the main components which play a key role in gravistimulation signalling in flowering shoots. © Springer Science+Business Media New York 2015. All right reserved.
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