חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Plasma membrane potential of the alga Dunaliella, and its relation to osmoregulatin
Year:
1990
Authors :
אורן-שמיר, מיכל
;
.
Volume :
93
Co-Authors:
Oren-Shamir, M., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Pick, U., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Avron, M., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
403
To page:
408
(
Total pages:
6
)
Abstract:
A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halotolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K+ and valinomycin, or a protonophore, was taken as a measure of the preexisting potential. A resting potential of -85 to -100 millivolts (negative inside) was calculated. Following a hypertonic shock, the plasma membrane was rapidly hyperpolarized. This hyperpolarization was transient, and the algae resumed their resting potential about 30 minutes after the shock. The resting plasma membrane potential was decreased by vanadate and is concluded to be generated mostly by the plasma membrane ATPase of Dunaliella. The transient hyperpolarization following a hypertonic shock indicates, therefore, a transient activation of the ATPase. This is further corroborated by a rapid transient decrease in the intracellular ATP following a hypertonic shock and its inhibition by vanadate. It is suggested that activation of the plasma membrane ATPase may be the trigger for osmoregulation in Dunaliella.
Note:
Related Files :
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29482
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
Scientific Publication
Plasma membrane potential of the alga Dunaliella, and its relation to osmoregulatin
93
Oren-Shamir, M., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Pick, U., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Avron, M., Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel
Plasma membrane potential of the alga Dunaliella, and its relation to osmoregulatin
A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halotolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K+ and valinomycin, or a protonophore, was taken as a measure of the preexisting potential. A resting potential of -85 to -100 millivolts (negative inside) was calculated. Following a hypertonic shock, the plasma membrane was rapidly hyperpolarized. This hyperpolarization was transient, and the algae resumed their resting potential about 30 minutes after the shock. The resting plasma membrane potential was decreased by vanadate and is concluded to be generated mostly by the plasma membrane ATPase of Dunaliella. The transient hyperpolarization following a hypertonic shock indicates, therefore, a transient activation of the ATPase. This is further corroborated by a rapid transient decrease in the intracellular ATP following a hypertonic shock and its inhibition by vanadate. It is suggested that activation of the plasma membrane ATPase may be the trigger for osmoregulation in Dunaliella.
Scientific Publication
נגישות
menu      
You may also be interested in