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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci
Year:
2001
Source of publication :
Phytopathology
Authors :
גנאים, מוראד
;
.
Volume :
91
Co-Authors:
Ghanim, M., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Morin, S., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Czosnek, H., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
188
To page:
196
(
Total pages:
9
)
Abstract:
Whiteflies (Bemisia tabaci, biotype B) were able to transmit Tomato yellow leaf curl virus (TYLCV) 8 h after they were caged with infected tomato plants. The spread of TYLCV during this latent period was followed in organs thought to be involved in the translocation of the virus in B. tabaci. After increasing acquisition access periods (AAPs) on infected tomato plants, the stylets, the head, the midgut, a hemolymph sample, and the salivary glands dissected from individual insects were subjected to polymerase chain reaction (PCR) without any treatment; the presence of TYLCV was assessed with virus-specific primers. TYLCV DNA was first detected in the head of B. tabaci after a 10-min AAP. The virus was present in the midgut after 40 min and was first detected in the hemolymph after 90 min. TYLCV was found in the salivary glands 5.5 h after it was first detected in the hemolymph. Subjecting the insect organs to immunocapture-PCR showed that the virus capsid protein was in the insect organs at the same time as the virus genome, suggesting that at least some TYLCV translocates as virions. Although females are more efficient as vectors than males, TYLCV was detected in the salivary glands of males and of females after approximately the same AAP.
Note:
Related Files :
Bemisia tabaci
biotype
Polymerase Chain Reaction
Tomato yellow leaf curl virus
virus DNA
virus infection
virus transmission
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29523
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
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Scientific Publication
Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci
91
Ghanim, M., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Morin, S., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Czosnek, H., Dept. of Field Crops and Genetics, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, Rehovot 76100, Israel
Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci
Whiteflies (Bemisia tabaci, biotype B) were able to transmit Tomato yellow leaf curl virus (TYLCV) 8 h after they were caged with infected tomato plants. The spread of TYLCV during this latent period was followed in organs thought to be involved in the translocation of the virus in B. tabaci. After increasing acquisition access periods (AAPs) on infected tomato plants, the stylets, the head, the midgut, a hemolymph sample, and the salivary glands dissected from individual insects were subjected to polymerase chain reaction (PCR) without any treatment; the presence of TYLCV was assessed with virus-specific primers. TYLCV DNA was first detected in the head of B. tabaci after a 10-min AAP. The virus was present in the midgut after 40 min and was first detected in the hemolymph after 90 min. TYLCV was found in the salivary glands 5.5 h after it was first detected in the hemolymph. Subjecting the insect organs to immunocapture-PCR showed that the virus capsid protein was in the insect organs at the same time as the virus genome, suggesting that at least some TYLCV translocates as virions. Although females are more efficient as vectors than males, TYLCV was detected in the salivary glands of males and of females after approximately the same AAP.
Scientific Publication
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