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פותח על ידי קלירמאש פתרונות בע"מ -
Linkage disequilibrium and spatial aggregation of genotypes in sexually reproducing populations of Erysiphe necator
Year:
2012
Source of publication :
Phytopathology
Authors :
פרנקל, עומר
;
.
Volume :
102
Co-Authors:
Brewer, M.T., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Department of Plant Pathology, University of Georgia, Athens, GA 30602, United States
Frenkel, O., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan 50250, Israel
Milgroom, M.G., Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, United States
Facilitators :
From page:
997
To page:
1005
(
Total pages:
9
)
Abstract:
Random mating and recombination in heterothallic ascomycetes should result in high genotypic diversity, 1:1 mating-type ratios, and random associations of alleles, or linkage equilibrium, at different loci. To test for random mating in populations of the grape powdery mildew fungus Erysiphe necator, we sampled isolates from vineyards of Vitis vinifera in Burdett, NY (NY09) and Winchester, VA (VA09) at the end of the epidemic in fall 2009. We also sampled isolates from the same Winchester, VA vineyard in spring 2010 at the onset of the next epidemic. Isolates were genotyped for mating type and 11 microsatellite markers. In the spring sample, which originated from ascospore infections, nearly every isolate had a unique genotype. In contrast, fall populations were less diverse. In all, 9 of 45 total genotypes in VA09 were represented by two or more isolates; 3 of 40 total genotypes in NY09 were represented by two or more isolates, with 1 genotype represented by 20 isolates. After clone correction, mating-type ratios in the three populations did not deviate from 1:1. However, even with clone correction, we detected significant linkage disequilibrium (LD) in all populations. Mantel tests detected positive correlations between genetic and physical distances within vineyards. Spatial autocorrelation showed aggregations up to 42 and 3 m in VA09 and NY09, respectively. Spatial autocorrelation most likely results from short dispersal distances. Overall, these results suggest that spatial genetic aggregation and clonal genotypes that arise during the asexual phase of the epidemic contribute to persistent LD even though populations undergo sexual reproduction annually. © 2012 The American Phytopathological Society.
Note:
Related Files :
article
Ascomycota
fungal DNA
gene linkage disequilibrium
Genetics
genotype
Mating systems
Polymerase Chain Reaction
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/PHYTO-11-11-0321
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29566
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
Scientific Publication
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