Cinnamon, Y., Department of Anatomy/Cell Biology, Hebrew University-Hadassah Med. Sch., PO Box 12272, Jerusalem 91120, Israel
Kahane, N., Department of Anatomy/Cell Biology, Hebrew University-Hadassah Med. Sch., PO Box 12272, Jerusalem 91120, Israel
Bachelet, I., Department of Anatomy/Cell Biology, Hebrew University-Hadassah Med. Sch., PO Box 12272, Jerusalem 91120, Israel
Kalcheim, C., Department of Anatomy/Cell Biology, Hebrew University-Hadassah Med. Sch., PO Box 12272, Jerusalem 91120, Israel

We have previously reported that the myotome is formed by a first wave of pioneer cells generated from all along the dorsomedial portion of the epithelial somite and a second wave of cells issued from all four edges of the dermomyotome. Cells from the extreme rostral and caudal edges directly generate myofibers that elongate towards the opposite pole of each segment and along the pre-existing myotomal scaffold. In contrast, cells from the dorsomedial and ventrolateral lips first reach the extreme edges and then contribute to myofiber formation. The mechanism by which these epithelial cells translocate remained unknonwn and was the goal of the present study. We have found that epithelial cells along the dorsomedial and ventrolateral lips of the dermomyotome first delaminate into the immediate underlayer of the corresponding lips, the sub-lip domain, then migrate longitudinally along this pathway until reaching the extreme edges from which they differentiate into myofibers. Cells of the sub-lip domain are negative for Pax3 and desmin but express MyoD, Myf5 and FREK, suggesting that they are specific myogenic progenitors.