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פותח על ידי קלירמאש פתרונות בע"מ -
Influence of group A streptococcal acid glycoprotein on expression of major virulence factors and internalization by epithelial cells
Year:
2003
Source of publication :
Microbial Pathogenesis
Authors :
סלע, שלמה
;
.
Volume :
35
Co-Authors:
Marouni, M.J., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Ziomek, E., Biotechnology Research Institute, National Research Council Canada, Montreal, Que. H4P 2R2, Canada
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel, Department of Food Sciences, Institute of Technology and Storage, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Facilitators :
From page:
63
To page:
72
(
Total pages:
10
)
Abstract:
A single transposon insertion upstream to the open-reading-frame identified as the streptococcal acid glycoprotein (sagp) gene rendered a Tn916 isolate of Streptococcus pyogenes with elevated susceptibility to internalization by the epithelial cells. The role of SAGP in S. pyogenes internalization was further studied using isogenic mutant containing an in-frame deletion within the sagp gene. The sagp mutant displayed slower growth-rate and showed 5-fold higher internalization efficiency than the parent strain. Transcription of sagp at the logarithmic phase, but not at the stationary phase of the growth was repressed by csrR, the global regulator gene. At the same time, mutation of the sagp gene partially decreased the transcription of hasA, a gene that is required for capsule synthesis. The mutation had no effect on transcription of the emm3 gene, encoding for the M protein. The most striking effect of the sagp mutation was a down-regulation of the streptococcal pyrogenic exotoxin B (SpeB) at both translational and transcriptional level. Treatment of the SAGP mutant cells with the exogenous mSpeB (mature protease) only partially reduced their susceptibility to internalization. The exogenous mSpeB was more effective in reducing the internalization efficiency of a speB mutant and brought it to the level observed for the parent strain. In overall, results show that CsrR, directly or indirectly, affects the expression of SAGP, and that the SAGP modulates expression of not only SpeB, but also other genes that facilitate S. pyogenes internalization. © 2003 Elsevier Ltd. All rights reserved.
Note:
Related Files :
Cysteine Endopeptidases
exotoxin
gene expression
Sequence Deletion
Synthesis
transposons
Virulence Factors
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0882-4010(03)00094-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29660
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:48
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Scientific Publication
Influence of group A streptococcal acid glycoprotein on expression of major virulence factors and internalization by epithelial cells
35
Marouni, M.J., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Ziomek, E., Biotechnology Research Institute, National Research Council Canada, Montreal, Que. H4P 2R2, Canada
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel, Department of Food Sciences, Institute of Technology and Storage, Volcani Center, P.O. Box 6, Beth-Dagan 50250, Israel
Influence of group A streptococcal acid glycoprotein on expression of major virulence factors and internalization by epithelial cells
A single transposon insertion upstream to the open-reading-frame identified as the streptococcal acid glycoprotein (sagp) gene rendered a Tn916 isolate of Streptococcus pyogenes with elevated susceptibility to internalization by the epithelial cells. The role of SAGP in S. pyogenes internalization was further studied using isogenic mutant containing an in-frame deletion within the sagp gene. The sagp mutant displayed slower growth-rate and showed 5-fold higher internalization efficiency than the parent strain. Transcription of sagp at the logarithmic phase, but not at the stationary phase of the growth was repressed by csrR, the global regulator gene. At the same time, mutation of the sagp gene partially decreased the transcription of hasA, a gene that is required for capsule synthesis. The mutation had no effect on transcription of the emm3 gene, encoding for the M protein. The most striking effect of the sagp mutation was a down-regulation of the streptococcal pyrogenic exotoxin B (SpeB) at both translational and transcriptional level. Treatment of the SAGP mutant cells with the exogenous mSpeB (mature protease) only partially reduced their susceptibility to internalization. The exogenous mSpeB was more effective in reducing the internalization efficiency of a speB mutant and brought it to the level observed for the parent strain. In overall, results show that CsrR, directly or indirectly, affects the expression of SAGP, and that the SAGP modulates expression of not only SpeB, but also other genes that facilitate S. pyogenes internalization. © 2003 Elsevier Ltd. All rights reserved.
Scientific Publication
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