Theoretical and Applied Genetics
Gentile, A., Istituto di Coltivazioni Arboree, University of Catania, Catania, 95123, Italy
Tribulato, E., Istituto di Coltivazioni Arboree, University of Catania, Catania, 95123, Italy
Deng, Z.N., Hunan Horticultural Institute, Changsha, China
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, P. O. Box 26, Rehovot, 76100, Israel
Fluhr, R., Department of Plant Genetics, The Weizmann Institute of Science, P. O. Box 26, Rehovot, 76100, Israel
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan, 50250, Israel
Phoma tracheiphila is the causative agent of the disease mal secco. Citrus cultivars differ substantially in respect to their sensitivity to the pathogen P. tracheiphila and its toxin. Some cultivars (e.g., 'Femminello' lemon) are inherently sensitive while others (e.g., 'Tarocco' orange) are tolerant. Cell lines derived from nucellar tissue of 'Femminello', 'Tarocco' and a cell line selected for tolerance to the fungal toxin ('Femminello-S') were used to study host-pathogen interaction. Our results showed that calli or conditioned media of 'Tarocco' and 'Femminello-S' inhibited the size of co-cultivated fungal colonies when compared to 'Femminello'. In addition, conditioned medium of 'Tarocco' as well as 'FemminelloS', but not 'Femminello', promoted bursting of hyphal tips. A ten-fold increase in chitinase and glucanase enzymatic activity, as evaluated by radiometric assay and laminarin hydrolysis respectively, was detected in 'Femminello-S' extracellular extracts as compared to 'Femminello'. An increase in chitinase was also shown by immunoblot analysis. Our findings suggest a positive correlation between the presence of chitinase and glucanase in the conditioned media of the cultured cells and the tolerance of those cells to P. tracheiphila toxin. © 1993 Springer-Verlag.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Nucellar callus of 'Femminello' lemon, selected for tolerance to Phoma tracheiphila toxin, shows enhanced release of chitinase and glucanase into the culture medium
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Gentile, A., Istituto di Coltivazioni Arboree, University of Catania, Catania, 95123, Italy
Tribulato, E., Istituto di Coltivazioni Arboree, University of Catania, Catania, 95123, Italy
Deng, Z.N., Hunan Horticultural Institute, Changsha, China
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, P. O. Box 26, Rehovot, 76100, Israel
Fluhr, R., Department of Plant Genetics, The Weizmann Institute of Science, P. O. Box 26, Rehovot, 76100, Israel
Vardi, A., Institute of Horticulture, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan, 50250, Israel
Nucellar callus of 'Femminello' lemon, selected for tolerance to Phoma tracheiphila toxin, shows enhanced release of chitinase and glucanase into the culture medium
Phoma tracheiphila is the causative agent of the disease mal secco. Citrus cultivars differ substantially in respect to their sensitivity to the pathogen P. tracheiphila and its toxin. Some cultivars (e.g., 'Femminello' lemon) are inherently sensitive while others (e.g., 'Tarocco' orange) are tolerant. Cell lines derived from nucellar tissue of 'Femminello', 'Tarocco' and a cell line selected for tolerance to the fungal toxin ('Femminello-S') were used to study host-pathogen interaction. Our results showed that calli or conditioned media of 'Tarocco' and 'Femminello-S' inhibited the size of co-cultivated fungal colonies when compared to 'Femminello'. In addition, conditioned medium of 'Tarocco' as well as 'FemminelloS', but not 'Femminello', promoted bursting of hyphal tips. A ten-fold increase in chitinase and glucanase enzymatic activity, as evaluated by radiometric assay and laminarin hydrolysis respectively, was detected in 'Femminello-S' extracellular extracts as compared to 'Femminello'. An increase in chitinase was also shown by immunoblot analysis. Our findings suggest a positive correlation between the presence of chitinase and glucanase in the conditioned media of the cultured cells and the tolerance of those cells to P. tracheiphila toxin. © 1993 Springer-Verlag.
Scientific Publication