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פותח על ידי קלירמאש פתרונות בע"מ -
Taxol-induced apoptosis in human SKOV3 ovarian and MCF7 breast carcinoma cells is caspase-3 and caspase-9 independent
Year:
2002
Source of publication :
Cell Death and Differentiation
Authors :
רבינסקי, טטיאנה
;
.
Volume :
9
Co-Authors:
Ofir, R., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Seidman, R., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Rabinski, T., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Krup, M., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Yavelsky, V., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Weinstein, Y., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Wolfson, M., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Facilitators :
From page:
636
To page:
642
(
Total pages:
7
)
Abstract:
Taxol is used in chemotherapy regimens against breast and ovarian cancer. Treatment of tumor model cell lines with taxol induces apoptosis, but exact mechanism is not sufficiently understood. Our results demonstrate that in response to taxol, various cell types differentially utilize distinct apoptotic pathways. Using MCF7 breast carcinoma cells transfected with caspase-3 gene, we showed that taxol-induced apoptosis occurred in the absence of caspase-3 and caspase-9 activation. Similar results were obtained with ovarian SKOV3 carcinoma cells, expressing high level of endogenous caspase-3. In contrast, staurosporine-induced apoptosis in these cells was accompanied by proteolytic cleavage of pro-caspase-3 and induction of caspase-3 enzymatic activity. The effect of taxol appears to be cell type-specific, since taxol-induced apoptosis in leukemia U937 cells involved caspase-3 activation step. We conclude that a unique caspase-3 and caspase-9 independent pathway is elicited by taxol to induce apoptosis in human ovarian and breast cancinoma cells.
Note:
Related Files :
apoptosis
breast tumor
Caspase-3
Caspase-9
Female
Genetics
metabolism
Pathology
עוד תגיות
תוכן קשור
More details
DOI :
10.1038/sj/cdd/4401012
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29704
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:48
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Scientific Publication
Taxol-induced apoptosis in human SKOV3 ovarian and MCF7 breast carcinoma cells is caspase-3 and caspase-9 independent
9
Ofir, R., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Seidman, R., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Rabinski, T., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Krup, M., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Yavelsky, V., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Weinstein, Y., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Wolfson, M., Department of Microbiology/Immunol., Faculty of the Health Sciences, Ben Gurion University of the Negev, P.O.B. 653, Beer Sheva 84105, Israel
Taxol-induced apoptosis in human SKOV3 ovarian and MCF7 breast carcinoma cells is caspase-3 and caspase-9 independent
Taxol is used in chemotherapy regimens against breast and ovarian cancer. Treatment of tumor model cell lines with taxol induces apoptosis, but exact mechanism is not sufficiently understood. Our results demonstrate that in response to taxol, various cell types differentially utilize distinct apoptotic pathways. Using MCF7 breast carcinoma cells transfected with caspase-3 gene, we showed that taxol-induced apoptosis occurred in the absence of caspase-3 and caspase-9 activation. Similar results were obtained with ovarian SKOV3 carcinoma cells, expressing high level of endogenous caspase-3. In contrast, staurosporine-induced apoptosis in these cells was accompanied by proteolytic cleavage of pro-caspase-3 and induction of caspase-3 enzymatic activity. The effect of taxol appears to be cell type-specific, since taxol-induced apoptosis in leukemia U937 cells involved caspase-3 activation step. We conclude that a unique caspase-3 and caspase-9 independent pathway is elicited by taxol to induce apoptosis in human ovarian and breast cancinoma cells.
Scientific Publication
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