אסיף מאגר המחקר החקלאי

Direct evidence for the lack of methylation of two pulse labeled plant RNAs

Year:

1974

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Volume :

15

Co-Authors:

Posner, H.B., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Rosner, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel

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Abstract:

A product of the processing of precursor rRNA ("excess" RNA) has been indirectly found to be unmethylated in mammalian systems, but direct measurement was precluded because of its instability. The "excess" RNA of duckweeds is relatively stable allowing direct estimation of its methylation by polyacrylamide gel electrophoresis. The "excess" RNA with an apparent molecular weight 0.5×106 was unmethylated. A pulse labeled RNA with an apparent molecular weight of 1.2×106 (presumably from chloroplasts) was also unmethylated. Under similar conditions the presumed cytoplasmic rRNA precursors, and the mature cytoplasmic and chloroplast rRNAs were methylated. © 1974 Oxford University Press.

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