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פותח על ידי קלירמאש פתרונות בע"מ -
Establisitment and initial characterization of the ovine mammary epithelial cell line NISH
Year:
1998
Authors :
ברש, איתמר
;
.
גוטויין, אלישע
;
.
שני, משה
;
.
Volume :
34
Co-Authors:
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
326
To page:
332
(
Total pages:
7
)
Abstract:
Analysis of the molecular mechanisms involved in the differentiation and formation of the characteristic three-dimensional structures of the developing mammary gland of the major milk-producing livestock (ducts, end buds, and alveoli) requires in vitro model cell cultures. The few cell lines that have been established from dairy animals do not fully reproduce the entire program of mammary differentiation. Here we present the initial characterization of a unique mammary epithelial cell line derived spontaneously from midpregnant sheep (NISH). These cells form in vitro functional structures resembling ducts, lateral buds, and alveoli that secrete β-lactoglobulin (BLG) in an ECM (extracellular matrix)-dependent manner. Interestingly, the presence of growth hormone dramatically increased BLG secretion from NISH cells cultured on ECM. It appears that GH is required not only to establish the structural organization but also is continuously needed to maintain BLG expression. Stable transfection of NISH cells with BLG/Human Serum Albumin (HSA) hybrid gene constructs revealed that the relative level of expression was comparable to the in vivo secretion of HSA in transgenic mice carrying these gene sequences. No expression could be detected in cells transfected with hybrid genes carrying either HSA cDNA or the entire HSA gene, and HSA expression was dependent on the presence of intronic sequences. These results demonstrate that NISH cells may prove a useful tool for studying the differentiation and organogenesis of mammary epithelial cells under defined culture conditions. Furthermore, transfected NISH cells may be an alternative for the transgenic mouse model in evaluating the potential of gene constructs to be efficiently expressed in the mammary gland of transgenic farm animals.
Note:
Related Files :
Animal
Animals
Carcinogenesis
Female
gene expression
Genetics
metabolism
sheep
tissue culture
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29722
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
Establisitment and initial characterization of the ovine mammary epithelial cell line NISH
34
Ilan, N., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Barash, I., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Gootwine, E., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Shani, M., Institute of Animal Science, Volcani Center, Bet Dagan 50250, Israel
Establisitment and initial characterization of the ovine mammary epithelial cell line NISH
Analysis of the molecular mechanisms involved in the differentiation and formation of the characteristic three-dimensional structures of the developing mammary gland of the major milk-producing livestock (ducts, end buds, and alveoli) requires in vitro model cell cultures. The few cell lines that have been established from dairy animals do not fully reproduce the entire program of mammary differentiation. Here we present the initial characterization of a unique mammary epithelial cell line derived spontaneously from midpregnant sheep (NISH). These cells form in vitro functional structures resembling ducts, lateral buds, and alveoli that secrete β-lactoglobulin (BLG) in an ECM (extracellular matrix)-dependent manner. Interestingly, the presence of growth hormone dramatically increased BLG secretion from NISH cells cultured on ECM. It appears that GH is required not only to establish the structural organization but also is continuously needed to maintain BLG expression. Stable transfection of NISH cells with BLG/Human Serum Albumin (HSA) hybrid gene constructs revealed that the relative level of expression was comparable to the in vivo secretion of HSA in transgenic mice carrying these gene sequences. No expression could be detected in cells transfected with hybrid genes carrying either HSA cDNA or the entire HSA gene, and HSA expression was dependent on the presence of intronic sequences. These results demonstrate that NISH cells may prove a useful tool for studying the differentiation and organogenesis of mammary epithelial cells under defined culture conditions. Furthermore, transfected NISH cells may be an alternative for the transgenic mouse model in evaluating the potential of gene constructs to be efficiently expressed in the mammary gland of transgenic farm animals.
Scientific Publication
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