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פותח על ידי קלירמאש פתרונות בע"מ -
Cloning of a gene expressed during appressorium formation by Colletotrichum gloeosporioides and a marked decrease in virulence by disruption of this gene
Year:
1995
Source of publication :
Plant Cell
Authors :
פליישמן, משה
;
.
Volume :
7
Co-Authors:
Hwang, C.-S., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States, Johns Hopkins University, School of Medicine, Department of Biological Chemistry, 725 North Wolfe Street, Baltimore, MD 21205, United States
Flaishman, M.A., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States
Kolattukudy, P.E., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States, Ohio State University, Biotechnology Center, 206 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, United States
Facilitators :
From page:
183
To page:
193
(
Total pages:
11
)
Abstract:
Appressorium formation in germinating Colletotrichum gloeosporioides is induced by the surface wax of its host. One of the genes expressed uniquely in C. gloeosporioides during appressorium formation induced by the host signal has been designated cap20, and this gene and its cDNA were cloned and sequenced. Nucleotide sequences of both revealed an open reading frame that could encode a 183-amino acid polypeptide that did not have significant homology with any known proteins. Reverse transcriptase-polymerase chain reaction detected cap20 gene transcripts at the infection front on the surface and within tomato fruits infected by C. gloeosporioides. Gene-disrupted mutants incapable of expressing cap20 showed a drastically decreased virulence on avocado and tomato fruits. These results suggest that cap20 plays a significant role in the infection of the host.
Note:
Related Files :
Genetics
Microbiology
molecular genetics
mutation
Plant Disease
Plant Diseases
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29732
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
Cloning of a gene expressed during appressorium formation by Colletotrichum gloeosporioides and a marked decrease in virulence by disruption of this gene
7
Hwang, C.-S., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States, Johns Hopkins University, School of Medicine, Department of Biological Chemistry, 725 North Wolfe Street, Baltimore, MD 21205, United States
Flaishman, M.A., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States
Kolattukudy, P.E., Ohio State Biotechnology Center, Biochemistry Program, Ohio State University, Columbus, OH 43210, United States, Ohio State University, Biotechnology Center, 206 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, United States
Cloning of a gene expressed during appressorium formation by Colletotrichum gloeosporioides and a marked decrease in virulence by disruption of this gene
Appressorium formation in germinating Colletotrichum gloeosporioides is induced by the surface wax of its host. One of the genes expressed uniquely in C. gloeosporioides during appressorium formation induced by the host signal has been designated cap20, and this gene and its cDNA were cloned and sequenced. Nucleotide sequences of both revealed an open reading frame that could encode a 183-amino acid polypeptide that did not have significant homology with any known proteins. Reverse transcriptase-polymerase chain reaction detected cap20 gene transcripts at the infection front on the surface and within tomato fruits infected by C. gloeosporioides. Gene-disrupted mutants incapable of expressing cap20 showed a drastically decreased virulence on avocado and tomato fruits. These results suggest that cap20 plays a significant role in the infection of the host.
Scientific Publication
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