אסיף מאגר המחקר החקלאי

Strawberry‐cultivar Identification using Randomly Amplified Polymorphic DNA (RAPD) Markers

Year:

1994

Source of publication :

Authors :

Volume :

113

Co-Authors:

Gidoni, D., Department of Plant Genetics, Agricultural Research Organization (ARO), The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Rom, M., Department of Plant Genetics, Agricultural Research Organization (ARO), The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Kunik, T., Department of Plant Genetics, Agricultural Research Organization (ARO), The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Zur, M., Department of Agronomy and Natural Resources, Agricultural Research Organization (ARO), Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Izsak, E., Department of Vegetable Crops, Institute of Field and Garden Crops, Agricultural Research Organization ARO, Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics, Agricultural Research Organization (ARO), The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Firon, N., Department of Plant Genetics, Agricultural Research Organization (ARO), The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel

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Abstract:

Accurate and rapid cultivar identification is important for breeders'‐rights protection, especially for vegetatively propagated plants. The objective of this study was to test the feasibility of developing cultivar‐specific RAPD markers in commercial strawberries (Fragaria ananassa Dutch). Efforts were focused on distinguishing between two newly developed Volcani cultivars, ‘Ofra’ and ‘Dorit’, and six other cultivars, ‘Douglas’, ‘Chandler’, ‘Oso Grande’, ‘Dover’, ‘Nurit’ and ‘Parker’. Reproducible RAPD fingerprints were generated, each containing at least one polymorphic DNA product. A combination of 10 polymorphic DNA products exhibited cultivar‐specific patterns enabling the distinction between closely related varieties, such as ‘Ofra’ (which is the progeny of ‘Dorit’ and ‘Parker’) and ‘Dorit’ (which is the progeny of ‘Nurit’ and ‘Dover’). This study shows that RAPD markers can help in the protection of breeders’ rights to strawberry cultivars. Copyright © 1994, Wiley Blackwell. All rights reserved

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