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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Methods for detection of soilborne pathogens affecting strawberry in Israel
Year:
2009
Source of publication :
Acta Horticulturae
Authors :
פרימן, סטנלי
;
.
צוויבל, אאידה
;
.
Volume :
842
Co-Authors:
Zveibil, A., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Facilitators :
From page:
191
To page:
194
(
Total pages:
4
)
Abstract:
In Israel, strawberry is a winter producing crop cultivated as a fresh cropping system. Nuclear and foundation propagation material are indexed for viruses and checked for fungal pathogens twice annually, while spring-propagated field nurseries are monitored routinely for disease until summer transplanting to production fields. Various wilt pathogens affect the crop at different stages of propagation, however, the most vulnerable is at the field nursery stage where mother and daughter plants are exposed to outdoor, adverse conditions. Soilborne fungi such as Colletotrichum spp., Phytophthora spp., Rhizoctonia spp., Verticillium dahliae, Fusarium spp. and Macrophomina phaseolina are pathogens of the crop in Israel. Wilted plants are routinely sampled in the field by farmers and extension specialists from the Ministry of Agriculture and pathogens detected in the laboratory in Volcani Center. Initially, infected plant tissues were plated on selective medium for Phytophthora spp. and Fusarium spp., semi-selective medium for Colletotrichum spp. and V. dahliae, and non-selective PDA medium for Rhizoctonia spp. and M. phaseolina. Cultures isolated from the different media were inspected microscopically for morphological characters typical for the causal agent of disease. Colletotrichum and Phytophthora were further differentiated to the respective species (C. acutatum and C. gloeosporioides) and (P. cactorum and P. fragariae) according to specific-primer PCR amplification. Although Fusarium spp. were isolated in high numbers during the years 2005-2007, the predominant pathogen isolated from wilted plants during that period was M. phaseolina. A future research program based on pathogenicity tests and molecular gene sequences will determine whether the Fusarium spp. isolates are pathogenic to strawberry.
Note:
Related Files :
Colletotrichum
fungi
Fusarium
Phytophthora
Rhizoctonia
wilting
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29751
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
Scientific Publication
Methods for detection of soilborne pathogens affecting strawberry in Israel
842
Zveibil, A., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Methods for detection of soilborne pathogens affecting strawberry in Israel
In Israel, strawberry is a winter producing crop cultivated as a fresh cropping system. Nuclear and foundation propagation material are indexed for viruses and checked for fungal pathogens twice annually, while spring-propagated field nurseries are monitored routinely for disease until summer transplanting to production fields. Various wilt pathogens affect the crop at different stages of propagation, however, the most vulnerable is at the field nursery stage where mother and daughter plants are exposed to outdoor, adverse conditions. Soilborne fungi such as Colletotrichum spp., Phytophthora spp., Rhizoctonia spp., Verticillium dahliae, Fusarium spp. and Macrophomina phaseolina are pathogens of the crop in Israel. Wilted plants are routinely sampled in the field by farmers and extension specialists from the Ministry of Agriculture and pathogens detected in the laboratory in Volcani Center. Initially, infected plant tissues were plated on selective medium for Phytophthora spp. and Fusarium spp., semi-selective medium for Colletotrichum spp. and V. dahliae, and non-selective PDA medium for Rhizoctonia spp. and M. phaseolina. Cultures isolated from the different media were inspected microscopically for morphological characters typical for the causal agent of disease. Colletotrichum and Phytophthora were further differentiated to the respective species (C. acutatum and C. gloeosporioides) and (P. cactorum and P. fragariae) according to specific-primer PCR amplification. Although Fusarium spp. were isolated in high numbers during the years 2005-2007, the predominant pathogen isolated from wilted plants during that period was M. phaseolina. A future research program based on pathogenicity tests and molecular gene sequences will determine whether the Fusarium spp. isolates are pathogenic to strawberry.
Scientific Publication
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