נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats
Year:
1999
Source of publication :
Journal of Biological Chemistry
Authors :
מאירי, נעם
;
.
Volume :
274
Co-Authors:
Zhao, W., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Chen, H., Hypertension-Endocrine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, United States
Xu, H., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Moore, E., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Meiri, N., Institute of Animal Science, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Quon, M.J., Hypertension-Endocrine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Facilitators :
From page:
34893
To page:
34902
(
Total pages:
10
)
Abstract:
Evidence accumulated from clinical and basic research has indirectly implicated the insulin receptor (IR) in brain cognitive functions, including learning and memory (Wickelgren, I. (1998) Science 280, 517-519). The present study investigates correlative changes in IR expression, phosphorylation, and associated signaling molecules in the rat hippocampus following water maze training. Although the distribution of IR protein matched that of IR mRNA in most forebrain regions, a dissociation of the IR mRNA and protein expression patterns was found in the cerebellar cortex. After training, IR mRNA in the CA1 and dentate gyrus of the hippocampus was up-regulated, and there was increased accumulation of IR protein in the hippocampal crude synaptic membrane fraction. In the CA1 pyramidal neurons, changes in the distribution pattern of IR in particular cellular compartments, such as the nucleus and dendritic regions, was observed only in trained animals. Although IR showed a low level of in vivo tyrosine phosphorylation, an insulin-stimulated increase of in vitro Tyr phosphorylation of IR was detected in trained animals, suggesting that learning may induce IR functional changes, such as enhanced receptor sensitivity. Furthermore, a training-induced co-immunoprecipitation of IR with Shc-66 was detected, along with changes in in vivo Tyr phosphorylation of Shc and mitogen-activated protein kinase, as well as accumulation of Shc-66, Shc-52, and Grb-2 in hippocampal synaptic membrane fractions following training. These findings suggest that IR may participate in memory processing through activation of its receptor Tyr kinase activity, and they suggest possible engagement of Shc/Grb-2/Ras/mitogen-activated protein kinase cascades.
Note:
Related Files :
3T3 Cells
animal experiment
Animals
Cerebral Cortex
Male
mice
proteins
Receptor, IGF Type 1
עוד תגיות
תוכן קשור
More details
DOI :
10.1074/jbc.274.49.34893
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29794
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
You may also be interested in
Scientific Publication
Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats
274
Zhao, W., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Chen, H., Hypertension-Endocrine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, United States
Xu, H., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Moore, E., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Meiri, N., Institute of Animal Science, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Quon, M.J., Hypertension-Endocrine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, NINDS, National Institutes of Health, Bethesda, MD 20892, United States
Brain insulin receptors and spatial memory. Correlated changes in gene expression, tyrosine phosphorylation, and signaling molecules in the hippocampus of water maze trained rats
Evidence accumulated from clinical and basic research has indirectly implicated the insulin receptor (IR) in brain cognitive functions, including learning and memory (Wickelgren, I. (1998) Science 280, 517-519). The present study investigates correlative changes in IR expression, phosphorylation, and associated signaling molecules in the rat hippocampus following water maze training. Although the distribution of IR protein matched that of IR mRNA in most forebrain regions, a dissociation of the IR mRNA and protein expression patterns was found in the cerebellar cortex. After training, IR mRNA in the CA1 and dentate gyrus of the hippocampus was up-regulated, and there was increased accumulation of IR protein in the hippocampal crude synaptic membrane fraction. In the CA1 pyramidal neurons, changes in the distribution pattern of IR in particular cellular compartments, such as the nucleus and dendritic regions, was observed only in trained animals. Although IR showed a low level of in vivo tyrosine phosphorylation, an insulin-stimulated increase of in vitro Tyr phosphorylation of IR was detected in trained animals, suggesting that learning may induce IR functional changes, such as enhanced receptor sensitivity. Furthermore, a training-induced co-immunoprecipitation of IR with Shc-66 was detected, along with changes in in vivo Tyr phosphorylation of Shc and mitogen-activated protein kinase, as well as accumulation of Shc-66, Shc-52, and Grb-2 in hippocampal synaptic membrane fractions following training. These findings suggest that IR may participate in memory processing through activation of its receptor Tyr kinase activity, and they suggest possible engagement of Shc/Grb-2/Ras/mitogen-activated protein kinase cascades.
Scientific Publication
You may also be interested in