Co-Authors:
Amutha, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Muruganantham, M., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Ananthakrishnan, G., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Yablonsky, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Singer, S., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel
Abstract:
Regeneration in vitro from cotyledon explants of commercial squash (Cucurbita pepo L.) cultivars is generally efficient on Murashige and Skoog [Murashige, M., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15, 473-497] medium augmented with 4.4 μM benzyladenine. However, cotyledon explants from certain seed batches of cultivars Bareqet and Ma'yan could regenerate only buds, leaf primordia or very small shoots with storage for up to 2 years at 4 °C. Seed storage of cultivars Bareqet and Ma'yan at 4 °C for 6-8 years resulted in significant increases in shoot regeneration, shoot growth and explant growth, returning to the normal range of values for C. pepo. For example, for cv. Bareqet shoots regenerated per explant increased from 0.4 after storage for 2 years to 1.21 after storage for 8 years; shoot length increased from a mean of less than 2 mm after storage for 2 years to 22 mm after storage for 8 years. Additionally, the final explant fresh weight of cv. B increased from 181 mg after storage for 2 years, to 1389 mg after storage for 8 years. Similar responses were observed for seedling-derived explants of cv. Ma'yan following storage' for 2-7 years. However, total regeneration (number of explants regenerating buds, leaf primordia or shoots) was not affected by prolonged storage for either cultivar. This is the first report of stimulation of in vitro shoot regeneration of a seedling-derived organ caused by prolonged seed storage. Moreover, the great improvement in regeneration due to long-term seed storage provides a new mechanism for the understanding of non-repeatability of plant tissue culture results. © 2008 Elsevier B.V. All rights reserved.
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