אסיף מאגר המחקר החקלאי

Soybean Saponins: III. Fractionation And Characterization

Year:

1963

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Volume :

1

Co-Authors:

Gestetner, B., Faculty of Agriculturethe Hebrew University, Rehovoth, Israel
Ishaaya, I., Faculty of Agriculturethe Hebrew University, Rehovoth, Israel
Birk, Y., Faculty of Agriculturethe Hebrew University, Rehovoth, Israel
Bondi, A., Faculty of Agriculturethe Hebrew University, Rehovoth, Israel

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Abstract:

A method—consisting of column chromatography on acid washed Al2O3 and subsequent paper chromatography—for the fractionation of soybean saponin extract (SBSE) has been elaborated. SBSE could be separated into 5 fractions, the homogeneity of which has been established by ascending paper chromatography and horizontal paper electrophoresis. Four of these fractions were proved to be saponins by the following criteria: a) Haemolytic and foam‐forming abilities; b) Characteristic bitter taste; and c) Identification of the known soybean sapogenins A, B, C and D as well as reducing sugars, in the acid hydrolysates. A colorimetric method, based on a modified Lieberman‐Burchard reagent, for the quantitative determination of the various saponins present in SBSE has been developed. Saponin b, the strongest haemolytic agent of the four, is present only in small amounts in SBSE, whereas c, which composes 75% of SBSE, has moderate haemolytic activity. Since it has been found that the ratio of haemolytic and foam‐forming activity is inversely correlated, it seems that the two activities involve different active sites. Copyright © 1963 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

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