mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae

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mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae

Year: 

2007

Source of publication :

Molecular and Cellular Biology

Authors :

ציפור, גדי

.

Volume :

Co-Authors: 

Aronov, S., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gelin-Licht, R., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Haim, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Safran, E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel

From page: 

3441

To page: 

3455

(

Total pages: 

15

)

Link :

mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae

Abstract: 

Polarized growth in the budding yeast Saccharomyces cerevisiae depends upon the asymmetric localization and enrichment of polarity and secretion factors at the membrane prior to budding. We examined how these factors (i.e., Cdc42, Sec4, and Sro7) reach the bud site and found that their respective mRNAs localize to the tip of the incipient bud prior to nuclear division. Asymmetric mRNA localization depends upon factors that facilitate ASH1 mRNA localization (e.g., the 3′ untranslated region, She proteins 1 to 5, Puf6, actin cytoskeleton, and a physical association with She2). mRNA placement precedes protein enrichment and subsequent bud emergence, implying that mRNA localization contributes to polarization. Correspondingly, mRNAs encoding proteins which are not asymmetrically distributed (i.e., Snc1, Mso1, Tub1, Pex3, and Oxa1) are not polarized. Finally, mutations which affect cortical endoplasmic reticulum (ER) entry and anchoring in the bud (myo4Δ, sec3Δ, and srp101) also affect asymmetric mRNA localization. Bud-localized mRNAs, including ASH1, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast. Copyright © 2007, American Society for Microbiology. All Rights Reserved.