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פותח על ידי קלירמאש פתרונות בע"מ -
Tight regulation of allene oxide synthase (AOS) and allene oxide cyclase-3 (AOC3) promote Arabidopsis susceptibility to the root-knot nematode Meloidogyne javanica
Year:
2017
Authors :
אוזלבו, רחל
;
.
בוקי, פטריסיה
;
.
גורונג, פטה
;
.
הורוביץ, סיגל בראון
;
.
נאור, נוגה
;
.
Volume :
150
Co-Authors:
Naor, N., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Gurung, F.B., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ozalvo, R., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Bucki, P., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Sanadhya, P., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Miyara, S.B., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Facilitators :
From page:
149
To page:
165
(
Total pages:
17
)
Abstract:
Biosynthesis of the oxylipin jasmonic acid (JA) in Arabidopsis thaliana is catalyzed by a single allene oxide synthase (AOS)-encoding gene and four genes encoding four functional allene oxide cyclase (AOC) polypeptides (AOC1, AOC2, AOC3, and AOC4). To elucidate the biological activities of the JA pathway in regulating the plant defense response to plant-parasitic nematodes, transgenic lines carrying the GUS reporter gene under the control of individual AOC or AOS promoters were examined. Upon penetration by second-stage juveniles (J2 s), promoter activities of AOC1, AOC3 and AOC4 appeared in the root tip and root-elongation zone, with AOC3 demonstrating highest induction. At 5 days AOC3 activity continued to be highly pronounced in the stele and root cortex, associated with nematode invasion throughout gall initiation and maturation. AOS expression appeared 3 days postinfection and accompanied all later infection stages. Mutant lines were analyzed: disruption in AOS rendered plants more resistant to nematode infection, as reflected by the decreased number of females produced on this line; loss-of-function of AOC3 rendered plants more susceptible to nematode infection. Oxylipins derived from the 9- and 13-lipoxygenase pathways were assayed for their direct inhibitory activity toward M. javanica J2 s. Clear nematicidal activity of the bioactive 9- and 13-hydroperoxides was observed. Oxylipins produced by divinyl ether synthase, colneleic acid, colnelenic acid and ω5(Z)-etherolenic acid demonstrated strong inhibitory activity. These data, along with those of other assayed oxylipins, suggest that temporal and spatial fine tuning of the JA route allowing nematodes parasitism on plant host. © 2017 Koninklijke Nederlandse Planteziektenkundige Vereniging
Note:
Related Files :
Allene oxide cyclase
Allene oxide synthase
Jasmonate pathway
Meloidogyne
Oxylipins
Parasitism
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s10658-017-1261-2
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
29949
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
Scientific Publication
Tight regulation of allene oxide synthase (AOS) and allene oxide cyclase-3 (AOC3) promote Arabidopsis susceptibility to the root-knot nematode Meloidogyne javanica
150
Naor, N., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Gurung, F.B., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Ozalvo, R., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Bucki, P., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Sanadhya, P., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Miyara, S.B., Department of Entomology and the Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, HaMaccabim Road, P.O. Box 15159, Rishon Lezion, Israel
Tight regulation of allene oxide synthase (AOS) and allene oxide cyclase-3 (AOC3) promote Arabidopsis susceptibility to the root-knot nematode Meloidogyne javanica
Biosynthesis of the oxylipin jasmonic acid (JA) in Arabidopsis thaliana is catalyzed by a single allene oxide synthase (AOS)-encoding gene and four genes encoding four functional allene oxide cyclase (AOC) polypeptides (AOC1, AOC2, AOC3, and AOC4). To elucidate the biological activities of the JA pathway in regulating the plant defense response to plant-parasitic nematodes, transgenic lines carrying the GUS reporter gene under the control of individual AOC or AOS promoters were examined. Upon penetration by second-stage juveniles (J2 s), promoter activities of AOC1, AOC3 and AOC4 appeared in the root tip and root-elongation zone, with AOC3 demonstrating highest induction. At 5 days AOC3 activity continued to be highly pronounced in the stele and root cortex, associated with nematode invasion throughout gall initiation and maturation. AOS expression appeared 3 days postinfection and accompanied all later infection stages. Mutant lines were analyzed: disruption in AOS rendered plants more resistant to nematode infection, as reflected by the decreased number of females produced on this line; loss-of-function of AOC3 rendered plants more susceptible to nematode infection. Oxylipins derived from the 9- and 13-lipoxygenase pathways were assayed for their direct inhibitory activity toward M. javanica J2 s. Clear nematicidal activity of the bioactive 9- and 13-hydroperoxides was observed. Oxylipins produced by divinyl ether synthase, colneleic acid, colnelenic acid and ω5(Z)-etherolenic acid demonstrated strong inhibitory activity. These data, along with those of other assayed oxylipins, suggest that temporal and spatial fine tuning of the JA route allowing nematodes parasitism on plant host. © 2017 Koninklijke Nederlandse Planteziektenkundige Vereniging
Scientific Publication
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